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Literature summary extracted from

  • Shukla, S.; Shi, Q.; Maina, N.H.; Juvonen, M.; Maijatenkanen, M.; Goyal, A.
    Weissella confusa Cab3 dextransucrase: properties and in vitro synthesis of dextran and glucooligosaccharides (2014), Carbohydr. Polym., 101, 554-564.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.5 synthesis purified Weissella confusa Cab3 dextransucrase (WcCab3-DSR) is used for in vitro synthesis of dextran and glucooligosaccharides Weissella confusa

General Stability

EC Number General Stability Organism
2.4.1.5 0.1% glycerol and 1.0% Tween 80 enhance enzyme stability, enzyme half-life at 30°C increases from 10 h to 74 h and 59 h, respectively, stabilization by 0.01 mg/ml PEG-8000 Weissella confusa
2.4.1.5 dextran T-500 slightly stabilizes Weissella confusa
2.4.1.5 glutaraldehyde slightly destabilizes Weissella confusa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 EDTA 98% inhibition at 5 mM Weissella confusa
2.4.1.5 Mn2+ 42% inhibition at 10 mM Weissella confusa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Ca2+ activates by 20% at 1 mM Weissella confusa
2.4.1.5 Co2+ activates by 21% at 1 mM Weissella confusa
2.4.1.5 additional information poor effects by Zn2+, Mg2+, and Ni2+ at 1-2 mM Weissella confusa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Weissella confusa A0A0F6TP16
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 dextransucrase WcCab3-DSR 11fold by PEG fractionation Weissella confusa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 10.9
-
 purified enzyme, pH 5.4, 35°C Weissella confusa

Storage Stability

EC Number Storage Stability Organism
2.4.1.5 -20°C, purified enzyme, half-life: 37.9 days Weissella confusa
2.4.1.5 -4°C, purified enzyme, half-life: 69.4 days Weissella confusa
2.4.1.5 30°C, purified enzyme, half-life: 10.33 h Weissella confusa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 additional information Cab3 dextransucrase WcCab3-DSR is used for in vitro synthesis of dextran and glucooligosaccharides, product analysis by NMR and and mass spectrometry. Glucooligosaccarides are produced through the acceptor reaction with maltose Weissella confusa ?
-
 ?
2.4.1.5 sucrose + maltose
-
 Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1 glucooligosaccarides are produced through the acceptor reaction with maltose, a homologous series of isomaltooligosac-charides with reducing end maltose units ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n substrate is dextran Weissella confusa D-fructose + [(1->6)-alpha-D-glucosyl]n+1 the produced dextran confirmes the presence of main chain alpha-(1->6) linkages with only 3.0% of alpha-(1->3) branching, of which some are elongated ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 Cab3
-
 Weissella confusa
2.4.1.5 WcCab3-DSR
-
 Weissella confusa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 35
-
-
 Weissella confusa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 30
-
 glycerol and Tween 80 enhance enzyme stability, enzyme half-life at 30°C increases from 10 h to 74 h and 59 h, respectively Weissella confusa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
-
 Weissella confusa