BRENDA - Enzyme Database show

Extracellular nicotinamide phosphoribosyltransferase, a new cancer metabokine

Grolla, A.A.; Travelli, C.; Genazzani, A.A.; Sethi, J.K.; Br. J. Pharmacol. 173, 2182-2194 (2016)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.12
A244M
the mutation prevents inhibitor binding
Homo sapiens
2.4.2.12
D219A
inactive
Homo sapiens
2.4.2.12
D219S
the mutation leads to a switch in substrate affinity
Homo sapiens
2.4.2.12
G217R
the mutation prevents inhibitor binding
Homo sapiens
2.4.2.12
G384A
inactive
Homo sapiens
2.4.2.12
H191R
the mutation prevents inhibitor binding
Homo sapiens
2.4.2.12
H247A
inactive
Homo sapiens
2.4.2.12
H247E
the inactive mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization
Homo sapiens
2.4.2.12
K342R
inactive
Homo sapiens
2.4.2.12
Q388R
the mutation prevents dimerization
Homo sapiens
2.4.2.12
R311D
the inactive mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization
Homo sapiens
2.4.2.12
R392A
the inactive mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization
Homo sapiens
2.4.2.12
S199D
the mutation prevents dimerization and leads to loss of activity
Homo sapiens
2.4.2.12
S200D
the mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization. The mutation prevents dimerization
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
FK866
i.e. daporinad, (E)-N-[4-[1-(benzoyl)piperidin-4-yl]butyl]-3-pyridin-3-ylprop-2-enamide
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
extracellular
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Homo sapiens
P43490
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
3T3-L1 cell
-
Homo sapiens
-
2.4.2.12
adipocyte
-
Homo sapiens
-
2.4.2.12
amniotic epithelial cell
-
Homo sapiens
-
2.4.2.12
B-16 cell
-
Homo sapiens
-
2.4.2.12
breast cancer cell
-
Homo sapiens
-
2.4.2.12
BT-549 cell
-
Homo sapiens
-
2.4.2.12
cardiomyocyte
-
Homo sapiens
-
2.4.2.12
cervical cancer cell
-
Homo sapiens
-
2.4.2.12
chronic lymphocytic leukemia cell
-
Homo sapiens
-
2.4.2.12
colorectal cancer cell
-
Homo sapiens
-
2.4.2.12
DU-145 cell
-
Homo sapiens
-
2.4.2.12
fibroblast
-
Homo sapiens
-
2.4.2.12
glial cell
-
Homo sapiens
-
2.4.2.12
glioma cell
-
Homo sapiens
-
2.4.2.12
HCT-116 cell
-
Homo sapiens
-
2.4.2.12
HeLa cell
-
Homo sapiens
-
2.4.2.12
Hep-G2 cell
-
Homo sapiens
-
2.4.2.12
hepatoma cell
-
Homo sapiens
-
2.4.2.12
HIB-1B cell
-
Homo sapiens
-
2.4.2.12
HMCB cell
-
Homo sapiens
-
2.4.2.12
Huh-7 cell
-
Homo sapiens
-
2.4.2.12
HUVEC cell
-
Homo sapiens
-
2.4.2.12
LB-24_MEL cell
-
Homo sapiens
-
2.4.2.12
leucocyte
-
Homo sapiens
-
2.4.2.12
LS-180 cell
-
Homo sapiens
-
2.4.2.12
macrophage
-
Homo sapiens
-
2.4.2.12
MCF10A cell
-
Homo sapiens
-
2.4.2.12
MCF7 cell
-
Homo sapiens
-
2.4.2.12
MDA-MB-231 cell
-
Homo sapiens
-
2.4.2.12
MDA-MB-468 cell
-
Homo sapiens
-
2.4.2.12
melanocyte
-
Homo sapiens
-
2.4.2.12
melanoma cell
-
Homo sapiens
-
2.4.2.12
mesothelioma cell
-
Homo sapiens
-
2.4.2.12
MeWo cell
-
Homo sapiens
-
2.4.2.12
monocyte
-
Homo sapiens
-
2.4.2.12
neuroblastoma cell
-
Homo sapiens
-
2.4.2.12
neuron
-
Homo sapiens
-
2.4.2.12
pancreatic beta cell
-
Homo sapiens
-
2.4.2.12
pancreatic islet cell
-
Homo sapiens
-
2.4.2.12
PC-12 cell
-
Homo sapiens
-
2.4.2.12
peripheral blood lymphocyte
-
Homo sapiens
-
2.4.2.12
pre-B cell
-
Homo sapiens
-
2.4.2.12
prostate cancer cell
-
Homo sapiens
-
2.4.2.12
sebocyte
-
Homo sapiens
-
2.4.2.12
SH-SY5Y cell
-
Homo sapiens
-
2.4.2.12
SK-N-BE cell
-
Homo sapiens
-
2.4.2.12
SkMel-28 cell
-
Homo sapiens
-
2.4.2.12
T-47D cell
-
Homo sapiens
-
2.4.2.12
U-87 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
735872
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.2.12
A244M
the mutation prevents inhibitor binding
Homo sapiens
2.4.2.12
D219A
inactive
Homo sapiens
2.4.2.12
D219S
the mutation leads to a switch in substrate affinity
Homo sapiens
2.4.2.12
G217R
the mutation prevents inhibitor binding
Homo sapiens
2.4.2.12
G384A
inactive
Homo sapiens
2.4.2.12
H191R
the mutation prevents inhibitor binding
Homo sapiens
2.4.2.12
H247A
inactive
Homo sapiens
2.4.2.12
H247E
the inactive mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization
Homo sapiens
2.4.2.12
K342R
inactive
Homo sapiens
2.4.2.12
Q388R
the mutation prevents dimerization
Homo sapiens
2.4.2.12
R311D
the inactive mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization
Homo sapiens
2.4.2.12
R392A
the inactive mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization
Homo sapiens
2.4.2.12
S199D
the mutation prevents dimerization and leads to loss of activity
Homo sapiens
2.4.2.12
S200D
the mutant retains the same ability as wild type enzyme in STAT-3 activation, interleukin-6 secretion, macrophage survival following endoplasmic reticulum stress, and M2-polarization. The mutation prevents dimerization
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
FK866
i.e. daporinad, (E)-N-[4-[1-(benzoyl)piperidin-4-yl]butyl]-3-pyridin-3-ylprop-2-enamide
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
extracellular
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
3T3-L1 cell
-
Homo sapiens
-
2.4.2.12
adipocyte
-
Homo sapiens
-
2.4.2.12
amniotic epithelial cell
-
Homo sapiens
-
2.4.2.12
B-16 cell
-
Homo sapiens
-
2.4.2.12
breast cancer cell
-
Homo sapiens
-
2.4.2.12
BT-549 cell
-
Homo sapiens
-
2.4.2.12
cardiomyocyte
-
Homo sapiens
-
2.4.2.12
cervical cancer cell
-
Homo sapiens
-
2.4.2.12
chronic lymphocytic leukemia cell
-
Homo sapiens
-
2.4.2.12
colorectal cancer cell
-
Homo sapiens
-
2.4.2.12
DU-145 cell
-
Homo sapiens
-
2.4.2.12
fibroblast
-
Homo sapiens
-
2.4.2.12
glial cell
-
Homo sapiens
-
2.4.2.12
glioma cell
-
Homo sapiens
-
2.4.2.12
HCT-116 cell
-
Homo sapiens
-
2.4.2.12
HeLa cell
-
Homo sapiens
-
2.4.2.12
Hep-G2 cell
-
Homo sapiens
-
2.4.2.12
hepatoma cell
-
Homo sapiens
-
2.4.2.12
HIB-1B cell
-
Homo sapiens
-
2.4.2.12
HMCB cell
-
Homo sapiens
-
2.4.2.12
Huh-7 cell
-
Homo sapiens
-
2.4.2.12
HUVEC cell
-
Homo sapiens
-
2.4.2.12
LB-24_MEL cell
-
Homo sapiens
-
2.4.2.12
leucocyte
-
Homo sapiens
-
2.4.2.12
LS-180 cell
-
Homo sapiens
-
2.4.2.12
macrophage
-
Homo sapiens
-
2.4.2.12
MCF10A cell
-
Homo sapiens
-
2.4.2.12
MCF7 cell
-
Homo sapiens
-
2.4.2.12
MDA-MB-231 cell
-
Homo sapiens
-
2.4.2.12
MDA-MB-468 cell
-
Homo sapiens
-
2.4.2.12
melanocyte
-
Homo sapiens
-
2.4.2.12
melanoma cell
-
Homo sapiens
-
2.4.2.12
mesothelioma cell
-
Homo sapiens
-
2.4.2.12
MeWo cell
-
Homo sapiens
-
2.4.2.12
monocyte
-
Homo sapiens
-
2.4.2.12
neuroblastoma cell
-
Homo sapiens
-
2.4.2.12
neuron
-
Homo sapiens
-
2.4.2.12
pancreatic beta cell
-
Homo sapiens
-
2.4.2.12
pancreatic islet cell
-
Homo sapiens
-
2.4.2.12
PC-12 cell
-
Homo sapiens
-
2.4.2.12
peripheral blood lymphocyte
-
Homo sapiens
-
2.4.2.12
pre-B cell
-
Homo sapiens
-
2.4.2.12
prostate cancer cell
-
Homo sapiens
-
2.4.2.12
sebocyte
-
Homo sapiens
-
2.4.2.12
SH-SY5Y cell
-
Homo sapiens
-
2.4.2.12
SK-N-BE cell
-
Homo sapiens
-
2.4.2.12
SkMel-28 cell
-
Homo sapiens
-
2.4.2.12
T-47D cell
-
Homo sapiens
-
2.4.2.12
U-87 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
735872
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
2.4.2.12
Homo sapiens
enzyme expression is decreased by insulin, PI3K and AKT inhibitors, quercetin
down
2.4.2.12
Homo sapiens
enzyme expression is increased by Ox-LDL, CTRP3, glucose, rosiglitazone, adipocyte differentiation, ATP, LPS, CoCl2, ischaemia, OGD, anti-CD38 and differentiation in CCL, oxidative stress (H2O2), hypoxia, TNF-alpha, interleukin-1beta, starvation, and C-peptide
up
General Information
EC Number
General Information
Commentary
Organism
2.4.2.12
physiological function
the enzyme is involved in cancer
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.4.2.12
physiological function
the enzyme is involved in cancer
Homo sapiens
Expression (protein specific)
EC Number
Organism
Commentary
Expression
2.4.2.12
Homo sapiens
enzyme expression is decreased by insulin, PI3K and AKT inhibitors, quercetin
down
2.4.2.12
Homo sapiens
enzyme expression is increased by Ox-LDL, CTRP3, glucose, rosiglitazone, adipocyte differentiation, ATP, LPS, CoCl2, ischaemia, OGD, anti-CD38 and differentiation in CCL, oxidative stress (H2O2), hypoxia, TNF-alpha, interleukin-1beta, starvation, and C-peptide
up