Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Van Zyl, L.; Schubert, W.; Tuffin, M.; Cowan, D.
    Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus (2014), BMC Struct. Biol., 14, 21.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.1 expressed in Escherichia coli BL21(DE3) cells Gluconacetobacter diazotrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.1 to 1.69 A resolution, monoclinic space group C2 with cell dimensions: a = 129.1 A, b =141.0 A, c = 91.1 A, beta = 125.8°, with two monomers per asymmetric unit Gluconacetobacter diazotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.1 0.06
-
pyruvate pH 5.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 0.06
-
pyruvate at pH 5.0 and 25°C Gluconacetobacter diazotrophicus
4.1.1.1 0.6
-
pyruvate at pH 6.0 and 25°C Gluconacetobacter diazotrophicus
4.1.1.1 1.2
-
pyruvate pH 7.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 1.2
-
pyruvate at pH 7.0 and 25°C Gluconacetobacter diazotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.1 Mg2+ dependent on Gluconacetobacter diazotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.1 59200
-
-
Gluconacetobacter diazotrophicus
4.1.1.1 59200
-
x * 59200, calculated, x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
4.1.1.1 60000
-
x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
4.1.1.1 60000
-
x * 59200, calculated, x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.1 pyruvate Gluconacetobacter diazotrophicus
-
acetaldehyde + CO2
-
?
4.1.1.1 pyruvate Gluconacetobacter diazotrophicus ATCC 49037
-
acetaldehyde + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.1 Gluconacetobacter diazotrophicus A0A075Q354
-
-
4.1.1.1 Gluconacetobacter diazotrophicus A9H275
-
-
4.1.1.1 Gluconacetobacter diazotrophicus ATCC 49037 A9H275
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.1 Ni-NTA column chromatography Gluconacetobacter diazotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.1 0.15
-
substrate 2-oxo-4-methylpentanoate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 0.68
-
substrate 2-oxopentanoate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 12
-
substrate 2-oxobutanoate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 20
-
substrate pyruvate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 43
-
substrate pyruvate, pH 7.0, 25°C Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.1 2 pyruvate
-
Gluconacetobacter diazotrophicus (S)-acetolactate + CO2
-
?
4.1.1.1 2-oxo-4-methylpentanoate
-
Gluconacetobacter diazotrophicus 3-methylbutanaldehyde + CO2
-
?
4.1.1.1 2-oxo-4-methylpentanoate
-
Gluconacetobacter diazotrophicus ?
-
?
4.1.1.1 2-oxo-4-methylpentanoate
-
Gluconacetobacter diazotrophicus ATCC 49037 ?
-
?
4.1.1.1 2-Oxobutanoate
-
Gluconacetobacter diazotrophicus ?
-
?
4.1.1.1 2-Oxobutanoate
-
Gluconacetobacter diazotrophicus ATCC 49037 ?
-
?
4.1.1.1 2-oxobutanoate
-
Gluconacetobacter diazotrophicus propionaldehyde + CO2
-
?
4.1.1.1 2-Oxopentanoate
-
Gluconacetobacter diazotrophicus ?
-
?
4.1.1.1 2-Oxopentanoate
-
Gluconacetobacter diazotrophicus ATCC 49037 ?
-
?
4.1.1.1 2-oxopentanoate
-
Gluconacetobacter diazotrophicus butanaldehyde + CO2
-
?
4.1.1.1 3-phenyl-2-oxopropanoate
-
Gluconacetobacter diazotrophicus ?
-
?
4.1.1.1 additional information no substrate: indole-3-pyruvate Gluconacetobacter diazotrophicus ?
-
?
4.1.1.1 additional information no activity with benzoylformate, 3-hydroxyphenylpyruvate and indole-3-pyruvate Gluconacetobacter diazotrophicus ?
-
?
4.1.1.1 additional information no activity with benzoylformate, 3-hydroxyphenylpyruvate and indole-3-pyruvate Gluconacetobacter diazotrophicus ATCC 49037 ?
-
?
4.1.1.1 pyruvate
-
Gluconacetobacter diazotrophicus acetaldehyde + CO2
-
?
4.1.1.1 pyruvate
-
Gluconacetobacter diazotrophicus ATCC 49037 acetaldehyde + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.1 ? x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
4.1.1.1 ? x * 59200, calculated, x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
4.1.1.1 ? x * 59200, calaculated from amino acid sequence Gluconacetobacter diazotrophicus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.1 PDC
-
Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.1 45 50
-
Gluconacetobacter diazotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.1 60
-
half-life 18 min Gluconacetobacter diazotrophicus
4.1.1.1 60
-
the enzyme shows a half-life of18 min at 60°C Gluconacetobacter diazotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.1 5 5.5
-
Gluconacetobacter diazotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.1 thiamine diphosphate dependent on Gluconacetobacter diazotrophicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.1 Gluconacetobacter diazotrophicus calculated
-
5.8
4.1.1.1 Gluconacetobacter diazotrophicus calaculated from amino acid sequence
-
5.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.1 140
-
pyruvate pH 7.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 140
-
pyruvate at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
4.1.1.1 260
-
pyruvate at pH 6.0 and 25°C Gluconacetobacter diazotrophicus
4.1.1.1 1300
-
pyruvate pH 5.0, 25°C Gluconacetobacter diazotrophicus
4.1.1.1 1300
-
pyruvate at pH 5.0 and 25°C Gluconacetobacter diazotrophicus