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Literature summary extracted from

  • Egert, A.; Keller, F.; Peters, S.
    Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390) (2013), BMC Plant Biol., 13, 218.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.82 gene RFS5, functional recombinant expression in Escherichia coli strain BL21 codon plus Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.82 additional information construction of two independent loss-of-function mutants for gene RFS5, i.e. rs 5-1 and rs 5-2. Leaves of mutant plants fail to accumulate any raffinose under diverse abiotic stresses including water-deficit, high salinity, heat shock, and methyl viologen-induced oxidative stress. Correlated to the lack of raffinose under these abiotic stress conditions, both mutant plants lack the typical stress-induced raffinose synthase activity increase observed in the leaves of wild-type plants. The seeds of both mutant plants still contain raffinose Phenotypes, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.82 alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose Arabidopsis thaliana
-
 myo-inositol + raffinose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.82 Arabidopsis thaliana Q9FND9 gene RFS5, encoding stress-induced isoform RS5
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.82 leaf isozyme RS5 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.82 alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose
-
 Arabidopsis thaliana myo-inositol + raffinose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.82 raffinose synthase
-
 Arabidopsis thaliana
2.4.1.82 RS5
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.82 7.5
-
 assay at Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.4.1.82 Arabidopsis thaliana the enzyme is induced in leaves by abiotic stresses up

General Information

EC Number General Information Comment Organism
2.4.1.82 malfunction leaves of RFS5 mutant plants fail to accumulate any raffinose under diverse abiotic stresses including water-deficit, high salinity, heat shock, and methyl viologen-induced oxidative stress. Correlated to the lack of raffinose under these abiotic stress conditions, both mutant plants lack the typical stress-induced raffinose synthase activity increase observed in the leaves of wild-type plants Arabidopsis thaliana
2.4.1.82 metabolism the enzyme catalyzes the second step in raffinose biosynthesis Arabidopsis thaliana
2.4.1.82 physiological function the enzyme is responsible for low temperature-induced raffinose accumulation in Arabidopsis thaliana leaves Arabidopsis thaliana