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Literature summary extracted from

  • Mordukhova, E.A.; Kim, D.; Pan, J.G.
    Stabilized homoserine O-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone (2013), BMC Microbiol., 13, 179.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.46 gene metA, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain JW3973 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.46 A195T site-directed mutagenesis Escherichia coli
2.3.1.46 A200E site-directed mutagenesis Escherichia coli
2.3.1.46 D218G site-directed mutagenesis Escherichia coli
2.3.1.46 F247Y site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type Escherichia coli
2.3.1.46 I124L site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type Escherichia coli
2.3.1.46 I124L-I229Y site-directed mutagenesis, the mutant shows highly accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type Escherichia coli
2.3.1.46 I124L-I229Y-N267D site-directed mutagenesis, the mutant shows highly accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type Escherichia coli
2.3.1.46 I299Y site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type Escherichia coli
2.3.1.46 L110V site-directed mutagenesis Escherichia coli
2.3.1.46 additional information stabilized MetA mutant enzymes at least partially recover the growth defects of mutant Escherichia coli strains with deletions of either ATP-dependent proteases or the DnaK chaperone, increased accumulation of insoluble wild-type MetA in heat-stressed DELTAdnaK cells compared with the mutated I124L and I229Y enzymes, which have relative amounts of 57% and 33% of the wild-type enzyme, respectively Escherichia coli
2.3.1.46 Q96K site-directed mutagenesis, the mutant shows accelerated growth at 44°C in M9 glucose medium in contrast to the wild-type Escherichia coli
2.3.1.46 R160L site-directed mutagenesis Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.46 0.36
-
 succinyl-CoA recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 0.37
-
 succinyl-CoA recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 0.38
-
 succinyl-CoA recombinant His-tagged mutant I124L, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 0.83
-
 L-homoserine recombinant His-tagged mutant I124L, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 1.25
-
 L-homoserine recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 1.42
-
 L-homoserine recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.46 succinyl-CoA + L-homoserine Escherichia coli
-
 CoA + O-succinyl-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.46 Escherichia coli P07623 gene metA
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.46 additional information the wild-type strain W3110 does not grow at 45°C Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.46 succinyl-CoA + L-homoserine
-
 Escherichia coli CoA + O-succinyl-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.46 MetA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.46 36.72
-
 succinyl-CoA recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 36.72
-
 L-homoserine recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 38.59
-
 succinyl-CoA recombinant His-tagged mutant I124L, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 38.59
-
 L-homoserine recombinant His-tagged mutant I124L, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 39.28
-
 succinyl-CoA recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 39.28
-
 L-homoserine recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.46 succinyl-CoA
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.46 27.6
-
 L-homoserine recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 29.3
-
 L-homoserine recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 46.5
-
 L-homoserine recombinant His-tagged mutant I124L, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 99
-
 succinyl-CoA recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 102
-
 succinyl-CoA recombinant His-tagged mutant I124L, pH and temperature not specified in the publication Escherichia coli
2.3.1.46 109
-
 succinyl-CoA recombinant His-tagged mutant I229Y, pH and temperature not specified in the publication Escherichia coli