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Literature summary extracted from

  • Rostinawati, T.; Riani, C.; Elfahmi, E.; Sumirtapura, Y.; Retnoningrum, D.
    Y93F substitution of cyclodextrin glucanotransferase from Bacillus sp. A2-5a and its enzyme characterization (2015), Biotechnology, 14, 181-187.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 mutant enzyme Y93F is expressed in Escherichia coli BL21(DE3) cells Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 Y93F the substitution causes no alpha-cyclodextrin formation, but produces 6% more beta-cyclodextrin, 16% less gamma-cyclodextrin, and decreases its kcat and kcat/Km values Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 76390
-
 x * 76390, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor Bacillus sp. (in: Bacteria)
-
 cyclodextrins
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor Bacillus sp. (in: Bacteria) A2-5a
-
 cyclodextrins
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. (in: Bacteria)
-
-
-
2.4.1.19 Bacillus sp. (in: Bacteria) A2-5a
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 Ni-NTA resin column chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) cyclodextrins
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) A2-5a cyclodextrins
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 ? x * 76390, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 cyclodextrin glucanotransferase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 7
-
-
 Bacillus sp. (in: Bacteria)