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Literature summary extracted from

  • Ishida, R.; Sakaguchi, K.; Matsuzaki, C.; Katoh, T.; Ishida, N.; Yamamoto, K.; Hisa, K.
    Levansucrase from Leuconostoc mesenteroides NTM048 produces a levan exopolysaccharide with immunomodulating activity (2016), Biotechnol. Lett., 38, 681-687.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 recombinant expression of His-tagged enzyme in Escherichia coli Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 Cu2+
-
 Leuconostoc mesenteroides
2.4.1.10 EDTA 97% inhibition Leuconostoc mesenteroides
2.4.1.10 Fe2+ strong inhibition Leuconostoc mesenteroides
2.4.1.10 K+
-
 Leuconostoc mesenteroides
2.4.1.10 Li+
-
 Leuconostoc mesenteroides
2.4.1.10 Mg2+
-
 Leuconostoc mesenteroides
2.4.1.10 Mn2+
-
 Leuconostoc mesenteroides
2.4.1.10 Na+
-
 Leuconostoc mesenteroides
2.4.1.10 Ni2+
-
 Leuconostoc mesenteroides
2.4.1.10 Zn2+
-
 Leuconostoc mesenteroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.10 Ca2+ activates, recombinant enzyme Leuconostoc mesenteroides
2.4.1.10 Co2+ activates strongly, recombinant enzyme Leuconostoc mesenteroides
2.4.1.10 Rb+ activates slightly, recombinant enzyme Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 additional information Leuconostoc mesenteroides levan synthesis from sucrose ?
-
 ?
2.4.1.10 additional information Leuconostoc mesenteroides NTM048 levan synthesis from sucrose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Leuconostoc mesenteroides
-
 isolated from fresh green peas
-
2.4.1.10 Leuconostoc mesenteroides NTM048
-
 isolated from fresh green peas
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and ultrafiltration Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 additional information levan synthesis from sucrose Leuconostoc mesenteroides ?
-
 ?
2.4.1.10 additional information measurement of sucrose hydrolysis Leuconostoc mesenteroides ?
-
 ?
2.4.1.10 additional information levan synthesis from sucrose Leuconostoc mesenteroides NTM048 ?
-
 ?
2.4.1.10 additional information measurement of sucrose hydrolysis Leuconostoc mesenteroides NTM048 ?
-
 ?
2.4.1.10 sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
-
 Leuconostoc mesenteroides D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
-
 ?
2.4.1.10 sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
-
 Leuconostoc mesenteroides NTM048 D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 LvnS
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 30
-
 recombinant enzyme Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 5.5 6 recombinant enzyme Leuconostoc mesenteroides

General Information

EC Number General Information Comment Organism
2.4.1.10 evolution the enzyme belongs to the glycoside hydrolase family GH68 Leuconostoc mesenteroides
2.4.1.10 physiological function Levansucrase from Leuconostoc mesenteroides NTM048 produces a levan exopolysaccharide with immunomodulating activity stimulating IgA production in mice Leuconostoc mesenteroides