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Literature summary extracted from

  • Prihanto, A.A.; Nonomura, Y.; Takagi, K.; Naohara, R.; Umekawa, M.; Wakayama, M.
    Novel properties of gamma-glutamyltransferase from Pseudomonas syringae with beta-aspartyltransferase activity (2015), Biotechnol. Lett., 37, 2255-2263.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.7 dithiothreitol slight inhibition at 5 mM Pseudomonas syringae
2.3.2.7 EDTA slight inhibition at 5 mM Pseudomonas syringae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.7 L-asparagine + hydroxylamine Pseudomonas syringae
-
 NH3 + beta-L-aspartylhydroxamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.7 Pseudomonas syringae
-
 pv. phaseolicola 1448A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.7 DEAE-Cellufine column chromatography, butyl Toyopearl column chromatography, and Hitrap CM column chromatography Pseudomonas syringae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.7 L-asparagine + hydroxylamine
-
 Pseudomonas syringae NH3 + beta-L-aspartylhydroxamate
-
 ?
2.3.2.7 L-asparagine + hydroxylamine 301% activity compared to gamma-glutamyl-4-nitroanilide, hydroxylamine is the only substrate acceptor Pseudomonas syringae NH3 + beta-L-aspartylhydroxamate
-
 ?
2.3.2.7 additional information no activity with glycylglycine, ethylamine, and methylamine Pseudomonas syringae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.7 BAT
-
 Pseudomonas syringae
2.3.2.7 BAT-GGT bifunctional enzyme Pseudomonas syringae
2.3.2.7 beta-aspartyl-gamma-glutamyltransferase bifunctional enzyme Pseudomonas syringae
2.3.2.7 beta-aspartyltransferase
-
 Pseudomonas syringae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.7 50
-
-
 Pseudomonas syringae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.7 30 60 more than 40% activity between 30 and 60°C Pseudomonas syringae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.7 30 70 after 10 min incubation at 30, 50, 50, 60 and 70°C, the enzyme shows 100, 95, 90, 85, and 60% residual activity, respectively Pseudomonas syringae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.7 9
-
-
 Pseudomonas syringae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.7 6 11 more than 40% activity between pH 6.0 and 11.0 Pseudomonas syringae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.2.7 5 11 after 10 min incubation at pH 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, and 11.0, the enzyme shows 30, 20, 55, 100, 95, 80, and 60% residual activity, respectively Pseudomonas syringae