EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.22 | gene dszA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Rhodococcus erythropolis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.22 | L346A | site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme | Rhodococcus erythropolis |
1.14.14.22 | Q345A | site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone | Rhodococcus erythropolis |
1.14.14.22 | T344A | site-directed mutagenesis, the mutant shows an unaltered substrate specificity compared to the wild-type enzyme | Rhodococcus erythropolis |
1.14.14.22 | T344A/Q345A | site-directed mutagenesis, the mutant strain can degrade octyl sulfide on which the wild-type strain cannot grow. the DszA change at residue 345 results in an altered C-S bond cleavage pattern of 3-methyl DBT sulfone | Rhodococcus erythropolis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.22 | Rhodococcus erythropolis | - |
gene dszA | - |
1.14.14.22 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
gene dszA | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.22 | 3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | activity of mutant Q345A | Rhodococcus erythropolis | ? + 2 FMN + H2O | - |
? | |
1.14.14.22 | 3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | activity of mutant Q345A | Rhodococcus erythropolis IGTS8 / ATCC 53968 | ? + 2 FMN + H2O | - |
? | |
1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
1.14.14.22 | dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
1.14.14.22 | additional information | no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes | Rhodococcus erythropolis | ? | - |
? | |
1.14.14.22 | additional information | no activity with 4,6-dibutyldibenzothiophene sulfone by wild-type and mutant enzymes | Rhodococcus erythropolis IGTS8 / ATCC 53968 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.22 | DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
1.14.14.22 | dibenzothiophene sulfone monooxygenase | - |
Rhodococcus erythropolis |
1.14.14.22 | dszA | - |
Rhodococcus erythropolis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.22 | FMNH2 | - |
Rhodococcus erythropolis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.22 | additional information | residue Q345 is involved in C-S bond cleavage specifically for alkylated dibenzothiophene sulfone | Rhodococcus erythropolis |