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Literature summary extracted from

  • Zhou, Y.; Wang, X.; Wei, W.; Xu, J.; Wang, W.; Xie, Z.; Zhang, Z.; Jiang, H.; Wang, Q.; Wei, C.
    A novel efficient beta-glucanase from a paddy soil microbial metagenome with versatile activities (2016), Biotechnol. Biofuels, 9, 36.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 0.62
-
 cellopentaose pH 7.0, 37°C uncultured bacterium
3.2.1.4 1.4
-
 4-nitrophenyl D-cellobioside pH 7.0, 37°C uncultured bacterium
3.2.1.4 5.1
-
 cellohexaose pH 7.0, 37°C uncultured bacterium
3.2.1.4 13.3
-
 Barley beta-glucan pH 7.0, 37°C uncultured bacterium
3.2.1.4 18.3
-
 cellotetraose pH 7.0, 37°C uncultured bacterium
3.2.1.4 20.8
-
 carboxymethyl cellulose pH 7.0, 37°C uncultured bacterium
3.2.1.4 28.3
-
 hydroxyethylcellulose pH 7.0, 37°C uncultured bacterium
3.2.1.4 47.3
-
 Laminarin pH 7.0, 37°C uncultured bacterium
3.2.1.4 151.7
-
 cellotriose pH 7.0, 37°C uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured bacterium F4YTG7 isolated from paddy soil
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-nitrophenyl D-cellobioside + H2O
-
 uncultured bacterium 4-nitrophenol + D-cellobiose
-
 ?
3.2.1.4 barley beta-glucan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 uncultured bacterium D-glucose + ?
-
 ?
3.2.1.4 cellohexaose + 5 H2O
-
 uncultured bacterium 6 D-glucose
-
 ?
3.2.1.4 cellopentaose + 4 H2O
-
 uncultured bacterium 5 D-glucose
-
 ?
3.2.1.4 cellotetraose + 3 H2O
-
 uncultured bacterium 4 D-glucose
-
 ?
3.2.1.4 cellotriose + 2 H2O
-
 uncultured bacterium 3 D-glucose
-
 ?
3.2.1.4 hydroxyethylcellulose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 laminarin + H2O
-
 uncultured bacterium 6 D-glucose
-
 ?
3.2.1.4 additional information Umcel9y-1 is an efficient endoglucanase and also exhibits high activities of exoglucanase and transglycosylation. The transglycosylation products of Umcel9y-1 including sophorose, laminaribiose, and gentiobiose, and transglycosylation are detected under all activated conditions. The decreasing order of catalytic efficiency for polysaccharides, cellooligosaccharides, and aryl-beta-glycosides is p-nitrophenyl-D-cellobioside, barley glucan, cellopentaose, cellotetraose, cellotriose, hydroxyethylcellulose, cellohexaose, laminarin, and carboxymethylcellulose, respectively uncultured bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 umcel9y-1
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 37
-
-
 uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 41.7
-
 cellohexaose pH 7.0, 37°C uncultured bacterium
3.2.1.4 42.3
-
 cellopentaose pH 7.0, 37°C uncultured bacterium
3.2.1.4 49.2
-
 carboxymethyl cellulose pH 7.0, 37°C uncultured bacterium
3.2.1.4 127.5
-
 Laminarin pH 7.0, 37°C uncultured bacterium
3.2.1.4 159
-
 cellotetraose pH 7.0, 37°C uncultured bacterium
3.2.1.4 185.6
-
 hydroxyethylcellulose pH 7.0, 37°C uncultured bacterium
3.2.1.4 466
-
 4-nitrophenyl D-cellobioside pH 7.0, 37°C uncultured bacterium
3.2.1.4 897
-
 cellotriose pH 7.0, 37°C uncultured bacterium
3.2.1.4 3276
-
 Barley beta-glucan pH 7.0, 37°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.5 7
-
 uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.4 2.3
-
 carboxymethyl cellulose pH 7.0, 37°C uncultured bacterium
3.2.1.4 2.7
-
 Laminarin pH 7.0, 37°C uncultured bacterium
3.2.1.4 5.6
-
 hydroxyethylcellulose pH 7.0, 37°C uncultured bacterium
3.2.1.4 5.9
-
 cellotriose pH 7.0, 37°C uncultured bacterium
3.2.1.4 8.2
-
 cellohexaose pH 7.0, 37°C uncultured bacterium
3.2.1.4 8.7
-
 cellotetraose pH 7.0, 37°C uncultured bacterium
3.2.1.4 68.7
-
 cellopentaose pH 7.0, 37°C uncultured bacterium
3.2.1.4 246
-
 Barley beta-glucan pH 7.0, 37°C uncultured bacterium
3.2.1.4 337
-
 4-nitrophenyl D-cellobioside pH 7.0, 37°C uncultured bacterium