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Literature summary extracted from

  • Choi, Y.H.; Kim, J.H.; Park, B.S.; Kim, B.G.
    Solubilization and iterative saturation mutagenesis of alpha1,3-fucosyltransferase from Helicobacter pylori to enhance its catalytic efficiency (2016), Biotechnol. Bioeng., 113, 1666-1675.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 expressed in Escherichia coli BW25113 (DE3) cells Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.152 A128N the mutant displays 3.4fold higher catalytic activity with D-lactose than the wild type enzyme Helicobacter pylori
2.4.1.152 A128N/H129E the mutant displays 6.5fold higher catalytic activity with D-lactose than the wild type enzyme Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.152 0.24
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.28
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.3
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.33
-
 GDP-beta-L-fucose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.37
-
 GDP-beta-L-fucose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.37
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 72
-
 D-lactose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 81
-
 D-lactose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 97
-
 D-lactose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 123
-
 D-lactose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 232
-
 D-lactose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 543
-
 D-lactose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-beta-L-fucose + D-lactose the enzyme has very low relative substrate specificity toward D-lactose Helicobacter pylori GDP + 3 L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-Glc
-
 ?
2.4.1.152 GDP-beta-L-fucose + N-acetyl-D-lactosamine
-
 Helicobacter pylori GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 alpha1,3-fucosyltransferase
-
 Helicobacter pylori
2.4.1.152 alpha1,3-FucT
-
 Helicobacter pylori
2.4.1.152 FutA
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.152 0.063
-
 GDP-beta-L-fucose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.113
-
 GDP-beta-L-fucose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.417
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.478
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 1.328
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 1.512
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 2.25
-
 D-lactose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 2.85
-
 D-lactose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 4
-
 D-lactose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 4.03
-
 D-lactose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 5.62
-
 D-lactose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 5.97
-
 D-lactose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.152 0.004
-
 D-lactose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.012
-
 D-lactose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.033
-
 D-lactose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.041
-
 D-lactose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.069
-
 D-lactose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.083
-
 D-lactose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.191
-
 GDP-beta-L-fucose wild type enzyme, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 0.308
-
 GDP-beta-L-fucose mutant enzyme A128N, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 1.125
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 1.992
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 4.742
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/S46F, at pH 7.6 and 37°C Helicobacter pylori
2.4.1.152 5.041
-
 GDP-beta-L-fucose mutant enzyme A128N/H129E/Y132I/S46F, at pH 7.6 and 37°C Helicobacter pylori