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Literature summary extracted from

  • Kostylev, M.; Alahuhta, M.; Chen, M.; Brunecky, R.; Himmel, M.E.; Lunin, V.V.; Brady, J.; Wilson, D.B.
    Cel48A from Thermobifida fusca: structure and site directed mutagenesis of key residues (2014), Biotechnol. Bioeng., 111, 664-673.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.176 mutant D224N, to 1.6 A resolution. There is one molecule in the asymmetric unit in complex with a cellobiose and a cellohexaose molecule. The active site tunnel entrance is enriched in aromatic residues. Only the aromatic residues located around the tunnel entrance appear to be important for the ability of Cel48A to access individual cellulose chains on bacterial cellulose Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 F195A mutagenesis of active site tunnel residues, moderate reduction in activity Thermobifida fusca
3.2.1.176 W313A residue is important for processivity on bacterial cellulose Thermobifida fusca
3.2.1.176 W315A residue is important for processivity on bacterial cellulose Thermobifida fusca
3.2.1.176 Y213A mutagenesis of active site tunnel residues, moderate reduction in activity Thermobifida fusca
3.2.1.176 Y213A/S311A mutagenesis of active site tunnel residues, moderate reduction in activity Thermobifida fusca
3.2.1.176 Y213A/W313A mutagenesis of active site tunnel residues, moderate reduction in activity Thermobifida fusca
3.2.1.176 Y97A mutagenesis of active site tunnel residues, moderate reduction in activity Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Thermobifida fusca Q47NH7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 bacterial cellulose + H2O
-
 Thermobifida fusca cellobiose + cellotriose no release of D-.glucose is observed ?
3.2.1.176 phosphoric acid-swollen cellulose + H2O
-
 Thermobifida fusca cellobiose + cellotriose no release of D-.glucose is observed ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel48A
-
 Thermobifida fusca
3.2.1.176 Tfu_1959
-
 Thermobifida fusca