Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Trudeau, D.L.; Lee, T.M.; Arnold, F.H.
    Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures (2014), Biotechnol. Bioeng., 111, 2390-2397.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 degradation use of mutant T57N/E53D/S79P/T80E/V101I/S133R/N155E/G189S/F191V/T233V/G239E/V265T/D271Y/G293A7S309W/S318P and previously engineered highly active, thermostable variants of the fungal cellobiohydrolases Cel6A and Cel7A to hydrolyzes cellulose synergistically at an optimum temperature of 70°C over 60 h.The thermostable mixture produces three times as much total sugar as the best mixture of the wild type enzymes operating at their optimum temperature of 60°C Trichoderma reesei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Saccharomyces cerevisiae Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 T57N/E53D/S79P/T80E/V101I/S133R/N155E/G189S/F191V/T233V/G239E/V265T/D271Y/G293A7S309W/S318P thermostable mutant combining previously identified stabilizing mutations. Mutant has an optimal temperature 17°C higher than wild type and hydrolyzes 1.5 times as much cellulose over 60 h at its optimum temperature compared to the wild type enzyme at its optimal temperature Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Trichoderma reesei G0RB67
-
-
3.2.1.4 Trichoderma reesei QM6a G0RB67
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.4 avicel + H2O
-
 Trichoderma reesei QM6a ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 64
-
 wild-type Trichoderma reesei
3.2.1.4 81
-
 mutant T57N/E53D/S79P/T80E/V101I/S133R/N155E/G189S/F191V/T233V/G239E/V265T/D271Y/G293A/S309W/S318P Trichoderma reesei