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Literature summary extracted from

  • Li, R.; Zhang, T.; Jiang, B.; Mu, W.; Miao, M.
    Purification and characterization of an intracellular levansucrase derived from Bacillus methylotrophicus SK 21.002 (2015), Biotechnol. Appl. Biochem., 62, 815-822.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 Cu2+ inhibits transfructosylation and hydrolytic activities Bacillus velezensis
2.4.1.10 Fe2+ inhibits transfructosylation and hydrolytic activities Bacillus velezensis
2.4.1.10 Mn2+ inhibits only the hydrolytic activity Bacillus velezensis
2.4.1.10 N-bromosuccinimide
-
 Bacillus velezensis
2.4.1.10 Ni2+ inhibits transfructosylation and hydrolytic activities Bacillus velezensis
2.4.1.10 phenylmethanesulfonyl fluoride
-
 Bacillus velezensis
2.4.1.10 Zn2+ inhibits transfructosylation and hydrolytic activities Bacillus velezensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.10 additional information
-
 additional information Michaelis-Menten kinetics Bacillus velezensis
2.4.1.10 117.2
-
 sucrose pH 6.5, 40°C Bacillus velezensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 intracellular
-
 Bacillus velezensis 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.10 Ca2+ activates both transfructosylation and hydrolytic activities Bacillus velezensis
2.4.1.10 Mg2+ activates both transfructosylation and hydrolytic activities Bacillus velezensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.10 60000
-
 gel filtration Bacillus velezensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n Bacillus velezensis
-
 D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
 ?
2.4.1.10 sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n Bacillus velezensis SK 21.002
-
 D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Bacillus velezensis
-
-
-
2.4.1.10 Bacillus velezensis SK 21.002
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 native intracellular enzyme 11.8fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Bacillus velezensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.10 135.4
-
 purified enzyme, pH 6.5, 40°C Bacillus velezensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
-
 Bacillus velezensis D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
 ?
2.4.1.10 sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
-
 Bacillus velezensis SK 21.002 D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 monomer 1 * 60000, intracellular enzyme, SDS-PAGE Bacillus velezensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 40
-
 transfructosylation activity Bacillus velezensis
2.4.1.10 45
-
 hydrolytic activity Bacillus velezensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6.5
-
 transfructosylation and hydrolytic activity Bacillus velezensis