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Literature summary extracted from

  • Chen, Y.C.; Chen, W.T.; Liu, J.C.; Tsai, L.C.; Cheng, H.L.
    A highly active beta-glucanase from a new strain of rumen fungus Orpinomyces sp. Y102 exhibits cellobiohydrolase and cellotriohydrolase activities (2014), Biores. Technol., 170, 513-521.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91
-
 Orpinomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.91 additional information CelC7 is insoluble when expressed in Escherichia coli. Deletion of 17 or 105 residues from the N-terminus significantly improves its solubility Orpinomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 additional information
-
 Barley beta-glucan Km value 2.18 mg/ml, 105-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
3.2.1.91 additional information
-
 Barley beta-glucan Km value 3.03 mg/ml, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.91 51500
-
 x * 51500, SDS-PAGE Orpinomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Orpinomyces sp.
-
-
-
3.2.1.91 Orpinomyces sp. Y102
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.91 proteolytic modification sequence contains a signal peptide (residues 1-17) Orpinomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.91 20.3
-
 substrate acid-swollen avicel, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
3.2.1.91 26
-
 substrate carboxymethyl cellulose, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
3.2.1.91 4564
-
 substrate barley beta-glucan, 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 acid-swollen avicel + H2O
-
 Orpinomyces sp. cellobiose + cellotriose
-
 ?
3.2.1.91 acid-swollen avicel + H2O
-
 Orpinomyces sp. Y102 cellobiose + cellotriose
-
 ?
3.2.1.91 barley beta-glucan + H2O
-
 Orpinomyces sp. ?
-
 ?
3.2.1.91 barley beta-glucan + H2O
-
 Orpinomyces sp. Y102 ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O
-
 Orpinomyces sp. ?
-
 ?
3.2.1.91 carboxymethyl cellulose + H2O
-
 Orpinomyces sp. Y102 ?
-
 ?
3.2.1.91 cellohexaose + H2O
-
 Orpinomyces sp. cellobiose + cellotriose + cellotetraose enzyme does not release D-glucose ?
3.2.1.91 cellohexaose + H2O
-
 Orpinomyces sp. Y102 cellobiose + cellotriose + cellotetraose enzyme does not release D-glucose ?
3.2.1.91 additional information no substrates: avicel, soluble oat spelt xylan, Pachyman, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-galactopyranoside Orpinomyces sp. ?
-
 ?
3.2.1.91 additional information no substrates: avicel, soluble oat spelt xylan, Pachyman, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-galactopyranoside Orpinomyces sp. Y102 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 51500, SDS-PAGE Orpinomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CelC7
-
 Orpinomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 45
-
 17-residue N-terminal truncation mutant Orpinomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.91 30 50 more than 70% of maximum activity, 17-residue N-terminal truncation mutant Orpinomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.91 4223
-
 Barley beta-glucan 105-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.
3.2.1.91 4402
-
 Barley beta-glucan 17-residue N-terminal truncation mutant, pH 6.0, 45°C Orpinomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 6
-
-
 Orpinomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.91 5 11
-
 Orpinomyces sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.91 additional information
-
 Barley beta-glucan Km value is 2.18 mg/ml, pH 6.0, 45°C, 17-residue N-terminal truncation mutant Orpinomyces sp.