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Literature summary extracted from

  • Kang, H.K.; Ko, E.A.; Kim, J.H.; Kim, D.
    Molecular cloning and characterization of active truncated dextransucrase from Leuconostoc mesenteroides B-1299CB4 (2013), Bioprocess Biosyst. Eng., 36, 857-865.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.5 gene dsrE563, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS using expression vector pRSETC Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.5 additional information construction of two truncated derivative mutants DsrE563DCD2DGBD (DsrE563-1) and DsrE563DCD2DVR (DsrE563-2). Mutant DsrE563-1 with a deletion of 1620 amino acids from the C-terminus, and mutant DsrE563-2 with deletion of 1258 amino acids from the C-terminus and 349 amino acids from the N-terminus, are catalytically active synthesizing less-soluble dextran, mainly containing alpha-1,6 glucosidic linkage, the synthesized less-soluble dextran also has a branched alpha-1,3 linkage. Mutant DsrE563-2 shows 4.5fold higher dextransucrase activity than mutant DsrE563-1 and a higher acceptor reaction efficiency compared to the wild-type enzyme from Leuconostoc mesenteroides strain 512 FMCM when various mono- or disaccharides are used as acceptors Leuconostoc mesenteroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Ca2+ required Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 132900
-
 x * 313300, about, sequence calculation, x * 132900, about, mutant DsrE563-1, x * 136800, about, mutant DsrE563-2, sequence calculation, sequence calculation, x * 128000-146000, purified mutant DsrE563-2 exists in multiple molecular forms, SDS-PAGE Leuconostoc mesenteroides
2.4.1.5 136800
-
 x * 313300, about, sequence calculation, x * 132900, about, mutant DsrE563-1, x * 136800, about, mutant DsrE563-2, sequence calculation, sequence calculation, x * 128000-146000, purified mutant DsrE563-2 exists in multiple molecular forms, SDS-PAGE Leuconostoc mesenteroides
2.4.1.5 313300
-
 x * 313300, about, sequence calculation, x * 132900, about, mutant DsrE563-1, x * 136800, about, mutant DsrE563-2, sequence calculation, sequence calculation, x * 128000-146000, purified mutant DsrE563-2 exists in multiple molecular forms, SDS-PAGE Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n Leuconostoc mesenteroides B-1299CB4
-
 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides G8XR50 constitutive gene dsrE563
-
2.4.1.5 Leuconostoc mesenteroides B-1299CB4 G8XR50 constitutive gene dsrE563
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by anion exchange and hydrophobic interaction chromatography Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 additional information dextran product analysis by treatment with Penicillium dextranase and by thin layer chromatography, and by NMR spectroscopy Leuconostoc mesenteroides ?
-
 ?
2.4.1.5 additional information dextran product analysis by treatment with Penicillium dextranase and by thin layer chromatography, and by NMR spectroscopy Leuconostoc mesenteroides B-1299CB4 ?
-
 ?
2.4.1.5 sucrose + D-glucose
-
 Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1 products determined are leucrose, isomaltose, glucosyl leucroside, isomaltotriose, isomaltosyl leucroside, and isomaltotetrose ?
2.4.1.5 sucrose + D-glucose
-
 Leuconostoc mesenteroides B-1299CB4 D-fructose + [(1->6)-alpha-D-glucosyl]n+1 products determined are leucrose, isomaltose, glucosyl leucroside, isomaltotriose, isomaltosyl leucroside, and isomaltotetrose ?
2.4.1.5 sucrose + isomaltose
-
 Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1 products determined are leucroside, glucosyl leucroside, isomaltotriose, and isomaltotetraose ?
2.4.1.5 sucrose + isomaltose
-
 Leuconostoc mesenteroides B-1299CB4 D-fructose + [(1->6)-alpha-D-glucosyl]n+1 products determined are leucroside, glucosyl leucroside, isomaltotriose, and isomaltotetraose ?
2.4.1.5 sucrose + maltose
-
 Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1 products determined are panose, alpha-isomaltosyl-1,6-alpha-D-glucopyranosyl-1,4-D-glucopyranose, and alpha-isomaltotriosyl-1,6-alpha-D-glucopyranosyl-1,4-D-glucopyranose ?
2.4.1.5 sucrose + maltose
-
 Leuconostoc mesenteroides B-1299CB4 D-fructose + [(1->6)-alpha-D-glucosyl]n+1 products determined are panose, alpha-isomaltosyl-1,6-alpha-D-glucopyranosyl-1,4-D-glucopyranose, and alpha-isomaltotriosyl-1,6-alpha-D-glucopyranosyl-1,4-D-glucopyranose ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Leuconostoc mesenteroides D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.5 sucrose + [(1->6)-alpha-D-glucosyl]n
-
 Leuconostoc mesenteroides B-1299CB4 D-fructose + [(1->6)-alpha-D-glucosyl]n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 ? x * 313300, about, sequence calculation, x * 132900, about, mutant DsrE563-1, x * 136800, about, mutant DsrE563-2, sequence calculation, sequence calculation, x * 128000-146000, purified mutant DsrE563-2 exists in multiple molecular forms, SDS-PAGE Leuconostoc mesenteroides
2.4.1.5 More the enzyme contains two catalytic domains (CD1 and CD2). The glucan-binding domain is important for both enzyme expression and dextransucrase activity Leuconostoc mesenteroides

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 DsrE563
-
 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 28
-
 assay at Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.2
-
 assay at Leuconostoc mesenteroides

General Information

EC Number General Information Comment Organism
2.4.1.5 evolution sequence comparisons of enzyme DsrE563 and enzyme DsrE, the enzymes show differences in the sequence but similar properties Leuconostoc mesenteroides
2.4.1.5 additional information the glucan-binding domain is important for both enzyme expression and dextransucrase activity Leuconostoc mesenteroides