EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.82 | - |
Serratia marcescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.82 | additional information | separation of the 3''-adenylyltransferase-aminoglycoside and 6'-acetyltransferase domains of the bifunctional enzyme, and comparison of their activity to that of the full-length enzyme | Serratia marcescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.82 | 0.00055 | - |
amikacin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.00177 | - |
netilmicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.00202 | - |
kanamycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.0022 | - |
sisomicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.00387 | - |
netilmicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.0049 | - |
sisomicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.00504 | - |
gentamicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.0079 | - |
tobramycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.0143 | - |
gentamicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.0148 | - |
tobramycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.016 | - |
ribostamycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.016 | - |
kanamycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.023 | - |
neomycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.0321 | - |
neomycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.0693 | - |
ribostamycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.127 | - |
amikacin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.147 | - |
acetyl-CoA | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 0.215 | - |
acetyl-CoA | full length enzyme, pH 7.5, 25°C | Serratia marcescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.82 | Serratia marcescens | Q8VQN7 | bifunctional 3''-adenylyltransferase-aminoglycoside and 6'-acetyltransferase | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.82 | acetyl-CoA + amikacin | - |
Serratia marcescens | CoA + 6'-N-acetylamikacin | - |
? | |
2.3.1.82 | acetyl-CoA + gentamicin | - |
Serratia marcescens | CoA + 6'-N-acetylgentamicin | - |
? | |
2.3.1.82 | acetyl-CoA + kanamycin | - |
Serratia marcescens | CoA + 6'-N-acetylkanamycin | - |
? | |
2.3.1.82 | acetyl-CoA + neomycin | - |
Serratia marcescens | CoA + 6'-N-acetylneomycin | - |
? | |
2.3.1.82 | acetyl-CoA + netilmicin | - |
Serratia marcescens | CoA + 6'-N-acetylnetilmicin | - |
? | |
2.3.1.82 | acetyl-CoA + ribostamycin | - |
Serratia marcescens | CoA + 6'-N-acetylribostamycin | - |
? | |
2.3.1.82 | acetyl-CoA + sisomicin | - |
Serratia marcescens | CoA + 6'-N-acetylsisomicin | - |
? | |
2.3.1.82 | acetyl-CoA + tobramycin | - |
Serratia marcescens | CoA + 6'-N-acetyltobramycin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.82 | ANT(3'')-Ii/AAC(6')-IId | - |
Serratia marcescens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.82 | 1.11 | - |
gentamicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 1.62 | - |
acetyl-CoA | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 1.91 | - |
netilmicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 2.13 | - |
kanamycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 2.92 | - |
amikacin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 2.93 | - |
sisomicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 3.21 | - |
netilmicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 3.33 | - |
gentamicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 3.73 | - |
tobramycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 3.81 | - |
kanamycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 3.84 | - |
tobramycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 3.88 | - |
ribostamycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 5.35 | - |
sisomicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 5.7 | - |
ribostamycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 7.09 | - |
amikacin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 9.06 | - |
neomycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 11.1 | - |
neomycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 12.1 | - |
acetyl-CoA | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.82 | 8.4 | - |
acetyl-CoA | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 62.4 | - |
amikacin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 82 | - |
acetyl-CoA | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 82.2 | - |
ribostamycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 133 | - |
kanamycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 189 | - |
kanamycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 220 | - |
gentamicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 234 | - |
gentamicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 243 | - |
ribostamycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 252 | - |
tobramycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 346 | - |
neomycin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 394 | - |
neomycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 485 | - |
tobramycin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 829 | - |
netilmicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 1080 | - |
netilmicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 1090 | - |
sisomicin | isolated AAC(6') domain, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 1330 | - |
sisomicin | full length enzyme, pH 7.5, 25°C | Serratia marcescens | |
2.3.1.82 | 5250 | - |
amikacin | full length enzyme, pH 7.5, 25°C | Serratia marcescens |