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Literature summary extracted from

  • Bubka, M.; Link-Lenczowski, P.; Janik, M.; Pochec, E.; Litynska, A.
    Overexpression of N-acetylglucosaminyltransferases III and V in human melanoma cells. Implications for MCAM N-glycosylation (2014), Biochimie, 103, 37-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.144 gene MGAT3, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-III, the cells show increased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT5 gene, EC 2.4.1.155 Homo sapiens
2.4.1.155 gene MGAT5, real-time RT-PCR enzyme expression analysis, establishing of human melanoma cell line WM-266-4 with induced stable overexpression of GnT-V, the cells show ncreased amounts of bisected and beta1-6 branched N-glycans on melanoma cell adhesion molecule MCAM/MUC18, but do not display significant differences in viability and capabilities to migrate through an endothelial layer, coexpression with MGAT3 gene, EC 2.4.1.144 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.144 Golgi apparatus
-
 Homo sapiens 5794
-
2.4.1.155 Golgi apparatus
-
 Homo sapiens 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.144 Mn2+ required Homo sapiens
2.4.1.155 Mn2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.144 additional information Homo sapiens the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases ?
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R Homo sapiens
-
 UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R
-
 ?
2.4.1.155 additional information Homo sapiens the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases ?
-
 ?
2.4.1.155 UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R Homo sapiens
-
 UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.144 Homo sapiens Q09327
-
-
2.4.1.155 Homo sapiens Q09328
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.144 melanoma cell
-
 Homo sapiens
-
2.4.1.155 melanoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.144 additional information the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases Homo sapiens ?
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + beta-D-mannosyl-R
-
 Homo sapiens UDP + 4-(N-acetyl-beta-D-glucosaminyl)-beta-D-mannosyl-R
-
 ?
2.4.1.155 additional information the MCAM glycoprotein, which is a biomarker of cutaneous melanoma, as a potential target for overexpressed glycosyltransferases Homo sapiens ?
-
 ?
2.4.1.155 UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 Homo sapiens UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.144 GnT-III
-
 Homo sapiens
2.4.1.144 Golgi beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase III
-
 Homo sapiens
2.4.1.155 GnT-IV
-
 Homo sapiens
2.4.1.155 Golgi beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase V
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.144 30
-
 assay at Homo sapiens
2.4.1.155 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.144 6
-
 assay at Homo sapiens
2.4.1.155 6
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.144 physiological function N-acetylglucosaminyltransferase III is involved in formation of beta1,4 linkage between GlcNAc and beta-mannose residue within the core of oligosaccharide. The resulting bisected N-glycan acquires a characteristic conformation that renders inaccessibility to many other enzymes participating in biosynthetic pathways of hybrid and complex multiantennary N-glycans as well as to lectins Homo sapiens
2.4.1.155 physiological function N-acetylglucosaminyltransferase V catalyzes the synthesis of the beta1-6 bond between the sugar residue of the donor and alpha1,6-linked mannose of the core of N-glycan. Subsequently, the beta1-6 branch is formed and antennae are initiated Homo sapiens