EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.13 | expressed in Escherichia coli BL21(DE3)pLysS cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.13 | D81A | the mutant shows strongly decreased activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | D81E | the mutant shows strongly decreased activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | D81E/N321I | the mutant shows strongly decreased activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | N321A | the mutant shows strongly decreased activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | N321I | the mutant shows strongly decreased activity compared to the wild type enzyme | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | Mg2+ | hydrolysis activity of the wild type enzyme is inhibited by increasing concentrations of Mg2+ with IC50 values in the low millimolar range | Mycobacterium tuberculosis | |
2.3.3.13 | additional information | not inhibited by EDTA | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.097 | - |
acetyl-CoA | mutant enzyme D81A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.123 | - |
acetyl-CoA | mutant enzyme N321A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.163 | - |
acetyl-CoA | mutant enzyme D81E/N321I, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.233 | - |
acetyl-CoA | mutant enzyme D81E, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.253 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.262 | - |
acetyl-CoA | mutant enzyme N321I, at pH 7.5 and 25°C | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 72000 | - |
2 * 72000, SDS-PAGE | Mycobacterium tuberculosis |
2.3.3.13 | 171000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis | - |
(2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | HisTrap column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis | (2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.13 | homodimer | 2 * 72000, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.13 | alpha-IPMS | - |
Mycobacterium tuberculosis |
2.3.3.13 | Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.05 | - |
acetyl-CoA | mutant enzyme D81A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.12 | - |
acetyl-CoA | mutant enzyme D81E, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.13 | - |
acetyl-CoA | mutant enzyme D81E/N321I, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.15 | - |
acetyl-CoA | mutant enzyme N321A, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.15 | - |
acetyl-CoA | mutant enzyme N321I, at pH 7.5 and 25°C | Mycobacterium tuberculosis | |
2.3.3.13 | 0.32 | - |
acetyl-CoA | wild type enzyme, at pH 7.5 and 25°C | Mycobacterium tuberculosis |