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Literature summary extracted from

  • Arioez, C.; Goetzke, H.; Lindholm, L.; Eriksson, J.; Edwards, K.; Daley, D.O.; Barth, A.; Wieslander, A.
    Heterologous overexpression of a monotopic glucosyltransferase (MGS) induces fatty acid remodeling in Escherichia coli membranes (2014), Biochim. Biophys. Acta, 1838, 1862-1870.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.336 expression in Escherichia coli Acholeplasma laidlawii
2.4.1.336 recombinant overexpression in Escherichia coli strain BL21-AI membranes resulting in an upregulated phospholipid synthesis and vesiculation, acyl chain ordering of lipids during enzyme MGS production, overview Acholeplasma laidlawii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.336 membrane
-
 Acholeplasma laidlawii 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.336 UDP-glucose + 1,2-diacyl-sn-glycerol Acholeplasma laidlawii
-
 UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.336 Acholeplasma laidlawii Q93P60
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.336 UDP-glucose + 1,2-diacyl-sn-glycerol
-
 Acholeplasma laidlawii UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.336 MGS
-
 Acholeplasma laidlawii
2.4.1.336 monoglucosyldiacylglycerol synthase
-
 Acholeplasma laidlawii

General Information

EC Number General Information Comment Organism
2.4.1.336 physiological function membrane protein monoglucosyldiacylglycerol synthase MGS is responsible for the creation of intracellular membranes when overexpressed in Escherichia coli. During 22 h of MGS induction, the lipid/protein ratio increases by 38% in MGS-expressing cells compared to control cells. Unsaturation levels remain constant for MGS cells (about 62%) but significantly decrease in control cells (from 61% to 36%). Cyclopropanated fatty acid content is lower in MGS producing cells while control cells have an increased cyclopropanation activity. Among all lipids, phosphatidylethanolamine is the most affected species in terms of cyclopropanation Acholeplasma laidlawii
2.4.1.336 physiological function role of enzyme MGS protein on stress regulation and cyclopropane fatty acid synthase activity. The fatty acid composition changes in enzyme MGS-overexpressing cells, overview Acholeplasma laidlawii