BRENDA - Enzyme Database

Characterization of Vis Toxin, a novel ADP-ribosyltransferase from Vibrio splendidus

Ravulapalli, R.; Lugo, M.R.; Pfoh, R.; Visschedyk, D.; Poole, A.; Fieldhouse, R.J.; Pai, E.F.; Merrill, A.R.; Biochemistry 54, 5920-5936 (2015)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
2.4.2.31
apoenzyme and in complex with NAD+ and inhibitors, vapor diffusion method
Vibrio splendidus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.31
0.272
-
Nomega-(ADP-D-ribosyl)-agmatine-L-arginine
pH and temperature not specified in the publication
Vibrio splendidus
2.4.2.31
0.276
-
NAD+
pH and temperature not specified in the publication
Vibrio splendidus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.2.31
NAD+ + agmatine
Vibrio splendidus
the enzyme labels L-arginine with ADP-ribose from the NAD(+) substrate at the amino nitrogen of the guanidinium side chain
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine
-
-
r
2.4.2.31
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine-L-arginine
Vibrio splendidus
-
NAD+ + agmatine L-arginine
-
-
r
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.2.31
Vibrio splendidus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.2.31
NAD+ + agmatine
the enzyme labels L-arginine with ADP-ribose from the NAD(+) substrate at the amino nitrogen of the guanidinium side chain
735686
Vibrio splendidus
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine
-
-
-
r
2.4.2.31
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine-L-arginine
-
735686
Vibrio splendidus
NAD+ + agmatine L-arginine
-
-
-
r
Synonyms
EC Number
Synonyms
Commentary
Organism
2.4.2.31
ADP-ribosyltransferase
-
Vibrio splendidus
2.4.2.31
Vis toxin
-
Vibrio splendidus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.4.2.31
apoenzyme and in complex with NAD+ and inhibitors, vapor diffusion method
Vibrio splendidus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.31
0.272
-
Nomega-(ADP-D-ribosyl)-agmatine-L-arginine
pH and temperature not specified in the publication
Vibrio splendidus
2.4.2.31
0.276
-
NAD+
pH and temperature not specified in the publication
Vibrio splendidus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.2.31
NAD+ + agmatine
Vibrio splendidus
the enzyme labels L-arginine with ADP-ribose from the NAD(+) substrate at the amino nitrogen of the guanidinium side chain
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine
-
-
r
2.4.2.31
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine-L-arginine
Vibrio splendidus
-
NAD+ + agmatine L-arginine
-
-
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.2.31
NAD+ + agmatine
the enzyme labels L-arginine with ADP-ribose from the NAD(+) substrate at the amino nitrogen of the guanidinium side chain
735686
Vibrio splendidus
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine
-
-
-
r
2.4.2.31
nicotinamide + Nomega-(ADP-D-ribosyl)-agmatine-L-arginine
-
735686
Vibrio splendidus
NAD+ + agmatine L-arginine
-
-
-
r