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Literature summary extracted from

  • Dunn, B.J.; Watts, K.R.; Robbins, T.; Cane, D.E.; Khosla, C.
    Comparative analysis of the substrate specificity of trans- versus cis-acyltransferases of assembly line polyketide synthases (2014), Biochemistry, 53, 3796-3806.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.B32 0.002
-
 methylmalonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B32 Saccharopolyspora erythraea Q03132 Q03132, i.e. modules 3 and 4, of 6-deoxyerythronolide B synthase, EC 2.3.1.94
-
2.3.1.94 Saccharopolyspora erythraea Q03131 and Q03132 and Q03133 Q03131, i.e. modules 1 and 2, Q03132, i.e. modules 3 and 4, Q03133, i.e. modules 5 and6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B32 ethylmalonyl-CoA + an [acyl-carrier protein]
-
 Saccharopolyspora erythraea CoA + a methylmalonyl-[acyl-carrier protein]
-
 ?
2.3.1.B32 malonyl-CoA + an [acyl-carrier protein]
-
 Saccharopolyspora erythraea CoA + a methylmalonyl-[acyl-carrier protein]
-
 ?
2.3.1.B32 methylmalonyl-CoA + an [acyl-carrier protein]
-
 Saccharopolyspora erythraea CoA + a methylmalonyl-[acyl-carrier protein]
-
 ?
2.3.1.B32 additional information the cis-acyltransferase domain from DEBS module 3 transfers a methylmalonyl extender unit from the corresponding carboxyacyl-CoA precursor onto the phosphopantetheine arm of the acyl carrier protein within the same module. The presence of the acyl carrier protein has little effect on the specificity of the cis-acyltransferase domain of 6-deoxyerythronolide B synthase for carboxyacyl-CoA substrates, but has a marked influence on the corresponding specificity parameters for the trans-acyltransferases from the disorazole and kirromycin synthases. Comparative analysis of the substrate specificity Saccharopolyspora erythraea ?
-
 ?
2.3.1.94 additional information the cis-acyltransferase domain from DEBS module 3 transfers a methylmalonyl extender unit from the corresponding carboxyacyl-CoA precursor onto the phosphopantetheine arm of the acyl carrier protein within the same module. The presence of the acyl carrier protein has little effect on the specificity of the cis-acyltransferase domain for carboxyacyl-CoA substrates, but has a marked influence on the corresponding specificity parameters for the trans-acyltransferases from the disorazole and kirromycin synthases. Comparative analysis of the substrate specificity Saccharopolyspora erythraea ?
-
 ?
2.3.1.94 propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+
-
 Saccharopolyspora erythraea 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.B32 0.033
-
 methylmalonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.B32 0.8
-
 ethylmalonyl-CoA below 0.8 per sec and mM, pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.B32 0.8
-
 malonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.B32 16.7
-
 methylmalonyl-CoA pH 7.6, temperature not specified in the publication Saccharopolyspora erythraea