EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.4.1 | expression in Escherichia coli | Geobacillus stearothermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.4.1 | H128A | mutation in subunit E1beta, residue His128 provides the proton required to protonate the incoming dithiolane ring in the reductive acetylation of the lipoyl goup | Geobacillus stearothermophilus |
1.2.4.1 | H271A | mutation in subunit E1alpha, residue His271 stabilizes the dianion formed during decarboxylation of the 2-oxo acid | Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 0.0011 | - |
pyruvate | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus | |
1.2.4.1 | 0.442 | - |
pyruvate | mutant H271A, pH 7.0, 30°C | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.1 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.4.1 | pyruvate + oxidized 2,6-dichlorophenolindophenol | - |
Geobacillus stearothermophilus | acetaldehyde + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 0.48 | - |
pyruvate | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus | |
1.2.4.1 | 3.8 | - |
pyruvate | mutant H271A, pH 7.0, 30°C | Geobacillus stearothermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | thiamine diphosphate | kcat/Km value for wild-type 20 per mM and s, for mutant H271A 950 per mM and s | Geobacillus stearothermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 9 | - |
pyruvate | mutant H271A, pH 7.0, 30°C | Geobacillus stearothermophilus | |
1.2.4.1 | 427 | - |
pyruvate | wild-type, pH 7.0, 30°C | Geobacillus stearothermophilus |