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Literature summary extracted from

  • Kumar, S.; Kaur, A.; Chattopadhyay, B.; Bachhawat, A.K.
    Defining the cytosolic pathway of glutathione degradation in Arabidopsis thaliana: role of the ChaC/GCG family of gamma-glutamyl cyclotransferases as glutathione-degrading enzymes and AtLAP1 as the Cys-Gly peptidase (2015), Biochem. J., 468, 73-85.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.9 expressed in Escherichia coli BL21 pLys2(DE3) cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.9 1.7
-
 glutathione isoform GGCT2;2, at pH 8.0 and 37°C Arabidopsis thaliana
4.3.2.9 4.96
-
 glutathione isoform GGCT2;3, at pH 8.0 and 37°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.9 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.9 Ni-NTA column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.9 5-L-glutamyl-L-alanine low activity compared to glutathione Arabidopsis thaliana 5-oxoproline + L-alanine
-
 ?
4.3.2.9 glutathione
-
 Arabidopsis thaliana 5-oxoproline + L-Cys-Gly
-
 ?
4.3.2.9 additional information no significant activity towards gamma-Glu-Cys Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.2.9 gamma-glutamyl cyclotransferases
-
 Arabidopsis thaliana
4.3.2.9 GGCT
-
 Arabidopsis thaliana
4.3.2.9 GGCT2;2 isoform Arabidopsis thaliana
4.3.2.9 GGCT2;3 isoform Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.9 0.113
-
 glutathione isoform GGCT2;3, at pH 8.0 and 37°C Arabidopsis thaliana
4.3.2.9 0.64
-
 glutathione isoform GGCT2;2, at pH 8.0 and 37°C Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.2.9 0.023
-
 glutathione isoform GGCT2;3, at pH 8.0 and 37°C Arabidopsis thaliana
4.3.2.9 0.378
-
 glutathione isoform GGCT2;2, at pH 8.0 and 37°C Arabidopsis thaliana