EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.57 | transduction of HEK-293T cells with an adenovirus encoding the enzyme and enzyme overexpression | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.57 | 3 acetyl-CoA + 2 spermine | Homo sapiens | - |
3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
2.3.1.57 | 3 acetyl-CoA + spermidine | Homo sapiens | - |
3 CoA + N1,N4,N8-triacetylspermidine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.57 | Homo sapiens | P21673 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.57 | 3 acetyl-CoA + 2 spermine | - |
Homo sapiens | 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine | - |
? | |
2.3.1.57 | 3 acetyl-CoA + spermidine | - |
Homo sapiens | 3 CoA + N1,N4,N8-triacetylspermidine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.57 | SAT1 | - |
Homo sapiens |
2.3.1.57 | spermidine/spermine N1-acetyltransferase 1 | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.57 | acetyl-CoA | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.57 | malfunction | key polyamine catabolic enzyme spermidine/spermine N1-acetyltransferase1 overexpression in HEK293T cells via adenoviral vector leads to a rapid depletion of spermidine and spermine, arrest in cell growth and a decline in cell viability. AdSAT1-transduced cells reveal morphological changes commonly associated with apoptosis, including cell shrinkage, nuclear fragmentation, mitochondrial alteration, vacuolization and membrane blebbing. As polyamine analogues, alpha-methylspermidine and N1,N12-dimethylspermine that are not substrates for SAT1 partially restore growth and prevent apoptosis of AdSAT1-transduced cells. Inhibition of polyamine oxidases does not restore the growth of AdSAT1-transduced cells or block apoptosis. AdSAT1-transduction causes apoptosis by an intrinsic mitochondrial pathway, release of cytochrome c from mitochondria to cytoplasm concomitant with a decrease in the mitochondrial fraction in AdSAT1-transduced cells | Homo sapiens |