EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.248 | F268V/D469Y/A513V/Y515S | mutant produces three times as much megalo-cycloisomaltooligosaccharides (10-12 glucose units) and 1.5 times as much total cycloisomaltooligosaccharides (7-12 glucose units) as compared with the wild-type. The modified product size specificity is attributable to the construction of novel substrate-binding sites in the B-2 substrate-binding site and reactivity is improved by mutation on subsite -3 on the catalytic domain | Niallia circulans |
2.4.1.248 | additional information | utational studies show that substrate-binding sites B-1 and B-2 contribute to recruiting substrate and maintaining product size respectively | Niallia circulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.248 | Niallia circulans | P94286 | - |
- |
2.4.1.248 | Niallia circulans T-3040 | P94286 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.248 | additional information | CITase from Bacillus circulans T-3040 produces mainly cycloisomaltooligosaccharides of 8 units with little megalo-cycloisomaltooligosaccharides with 10-12 glucose units | Niallia circulans | ? | - |
? | |
2.4.1.248 | additional information | CITase from Bacillus circulans T-3040 produces mainly cycloisomaltooligosaccharides of 8 units with little megalo-cycloisomaltooligosaccharides with 10-12 glucose units | Niallia circulans T-3040 | ? | - |
? | |
2.4.1.248 | soluble starch | - |
Niallia circulans | cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? | - |
? | |
2.4.1.248 | soluble starch | - |
Niallia circulans T-3040 | cyclo-(-6)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-4)-alpha-D-Glc-(1-) + ? | - |
? |