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Literature summary extracted from

  • Itoh, R.
    Purification and some properties of an IMP-specific 5'-nucleotidase from yeast (1994), Biochem. J., 298, 593-598.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.99 ADP 121% activity at 2.5 mM Saccharomyces cerevisiae
3.1.3.99 ATP 243% activity at 2.5 mM Saccharomyces cerevisiae
3.1.3.99 cyclic AMP 130% activity at 2.5 mM Saccharomyces cerevisiae
3.1.3.99 diadenosine pentaphosphate 252% activity at 2.5 mM Saccharomyces cerevisiae
3.1.3.99 Diadenosine tetraphosphate 283% activity at 2.5 mM Saccharomyces cerevisiae
3.1.3.99 diadenosine triphosphate 200% activity at 2.5 mM Saccharomyces cerevisiae
3.1.3.99 additional information not stimulated by AMP, GTP, CTP, adenosine 5'-[alpha,beta-methylene]diphosphate, and 2,3-bisphosphoglycerate Saccharomyces cerevisiae
3.1.3.99 UTP 139% activity at 2.5 mM Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.99 CTP 86% residual activity at 2.5 mM Saccharomyces cerevisiae
3.1.3.99 GTP 75% residual activity at 2.5 mM Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.99 Co2+ may substitute Mg2+ Saccharomyces cerevisiae
3.1.3.99 Mg2+ enzyme activity is absolutely dependent on bivalent metals with Mg2+ as most potent one Saccharomyces cerevisiae
3.1.3.99 Mn2+ may substitute Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.99 55400
-
 2 * 55400, SDS-PAGE Saccharomyces cerevisiae
3.1.3.99 220000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.99 IMP + H2O Saccharomyces cerevisiae highly specific substrate inosine + phosphate
-
 ?
3.1.3.99 additional information Saccharomyces cerevisiae no activity with AMP, dIMP, GMP, XMP, UMP, dTMP, CMP, 2'(3')-IMP, D-ribose 5-phosphate, phenyl phosphate, and beta-glyerophosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.99 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.99 ammonium sulfate precipitation and DEAE-Toyopearl column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.99 0.056
-
 crude extract, at pH 6.5 and 37°C Saccharomyces cerevisiae
3.1.3.99 102.5
-
 after 1830.4fold purification, at pH 6.5 and 37°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.99 IMP + H2O highly specific substrate Saccharomyces cerevisiae inosine + phosphate
-
 ?
3.1.3.99 additional information no activity with AMP, dIMP, GMP, XMP, UMP, dTMP, CMP, 2'(3')-IMP, D-ribose 5-phosphate, phenyl phosphate, and beta-glyerophosphate Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.99 homotetramer 2 * 55400, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.99 IMP-specific 5-nucleotidase
-
 Saccharomyces cerevisiae
3.1.3.99 Isn1
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.99 6 6.5 with 100 mM imidazole/HCl buffer or 100 mM sodium acetate buffer in the presence of 25 mM Mg2+ Saccharomyces cerevisiae