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Literature summary extracted from

  • Ghode, P.; Jobichen, C.; Ramachandran, S.; Bifani, P.; Sivaraman, J.
    Structural basis of mapping the spontaneous mutations with 5-flurouracil in uracil phosphoribosyltransferase from Mycobacterium tuberculosis (2015), Biochem. Biophys. Res. Commun., 467, 577-582.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 expressed in Escherichia coli Tuner (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.9 sitting drop vapor diffusion method, using 0.1 M sodium acetate trihydrate pH 4.6, 1.5 M sodium formate with a 2:1 reservoir-to-protein solution ratio at 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 25000
-
 4 * 25000, SDS-PAGE Mycobacterium tuberculosis
2.4.2.9 120000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 UMP + diphosphate
-
 r
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis H37Rv
-
 UMP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Mycobacterium tuberculosis P9WFF3
-
-
2.4.2.9 Mycobacterium tuberculosis H37Rv P9WFF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 MonoQ column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis UMP + diphosphate
-
 r
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis H37Rv UMP + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.9 homotetramer 4 * 25000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 UPRT
-
 Mycobacterium tuberculosis