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Literature summary extracted from

  • Liu, Y.; Feng, Y.; Wang, Y.; Li, X.; Cao, X.; Xue, S.
    Structural and biochemical characterization of MCAT from photosynthetic microorganism Synechocystis sp. PCC 6803 reveal its stepwise catalytic mechanism (2015), Biochem. Biophys. Res. Commun., 457, 398-403.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 gene fabD, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.39 F187A site-directed mutagenesis, the mutant shows loss of about 65-70% activity compared to the wild-type enzyme Synechocystis sp.
2.3.1.39 H188A site-directed mutagenesis, inactive mutant Synechocystis sp.
2.3.1.39 H87A site-directed mutagenesis, the mutant shows loss of about 80% activity compared to the wild-type enzyme Synechocystis sp.
2.3.1.39 Q10A site-directed mutagenesis, the mutant shows loss of about 70% activity compared to the wild-type enzyme Synechocystis sp.
2.3.1.39 R113A site-directed mutagenesis, inactive mutant Synechocystis sp.
2.3.1.39 S88T site-directed mutagenesis, the mutant initializes the reaction by H87 deprotonating S88T which is different from the wild-type, loss of 10% activity compared to the wild-type enzyme Synechocystis sp.
2.3.1.39 T56A site-directed mutagenesis, the mutant shows loss of about 60% activity compared to the wild-type enzyme Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] Synechocystis sp.
-
 CoA + a malonyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Synechocystis sp. P73242 gene fabD or slr2023
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] residues Arg113, Ser88 and His188 constitute the enzyme's catalytic triad, catalytic mechanism, detailed overview. Malonyl-CoA binds to MCAT, involving Ser88, Arg113, Met117 and Phe187. The second step forming an ACP-MCAT-malonyl intermediate is rate-limiting instead of the malonyl-CoA-MCAT intermediate formed in the first step. His87, Arg113 and Ser88 render different contributions for the two intermediates Synechocystis sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + an [acyl-carrier protein]
-
 Synechocystis sp. CoA + a malonyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 malonyl-coenzyme A: acyl-carrier protein transacylase
-
 Synechocystis sp.
2.3.1.39 MCAT
-
 Synechocystis sp.

General Information

EC Number General Information Comment Organism
2.3.1.39 additional information residues Arg113, Ser88 and His188 constitute cthe enzyme's atalytic triad. Structure homology modeling by molecular replacement using the enzyme from Escherichia coli, PDB: 1MLA, as a search model Synechocystis sp.