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Literature summary extracted from

  • Ihara, H.; Okada, T.; Ikeda, Y.
    Cloning, expression and characterization of Bombyx mori alpha1,6-fucosyltransferase (2014), Biochem. Biophys. Res. Commun., 450, 953-960.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.68 expressed in Sf21 insect cells Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.68 C46A the mutant shows wild type activity Bombyx mori
2.4.1.68 N183/341Q the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
2.4.1.68 N183D the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
2.4.1.68 N183D/N341D the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
2.4.1.68 N183Q the mutant shows wild type activity Bombyx mori
2.4.1.68 N341D the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
2.4.1.68 N341Q the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
2.4.1.68 R359A inactive Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.68 66000
-
 2 * 66000, SDS-PAGE Bombyx mori
2.4.1.68 120000
-
 gel filtration Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.68 GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine Bombyx mori
-
 GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.68 Bombyx mori A0A060PLD2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.68 glycoprotein Asn-183 and Asn-341 are the consensus sites for N-glycosylation Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.68 GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Bombyx mori GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.68 homodimer 2 * 66000, SDS-PAGE Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
2.4.1.68 alpha1,6-fucosyltransferase
-
 Bombyx mori
2.4.1.68 alpha1,6FucT
-
 Bombyx mori