Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yasuda, K.; Endo, M.; Ikushiro, S.; Kamakura, M.; Ohta, M.; Sakaki, T.
    UV-dependent production of 25-hydroxyvitamin D2 in the recombinant yeast cells expressing human CYP2R1 (2013), Biochem. Biophys. Res. Commun., 434, 311-315.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.24 functional recombinant expression of human CYP2R1 in Saccharomyces cerevisiae Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.24 microsome CYP2R1 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.24 calciferol + O2 + [reduced NADPH-hemoprotein reductase] Homo sapiens enzyme CYP2R1 hydroxylates vitamin D2 at position C-25 position 25-hydroxy-vitamin D2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.24 calciol + O2 + [reduced NADPH-hemoprotein reductase] Homo sapiens enzyme CYP2R1 hydroxylates vitamin D3 at position C-25 position calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.24 Homo sapiens Q6VVX0 gene CYP2R1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.24 calciferol + O2 + [reduced NADPH-hemoprotein reductase] enzyme CYP2R1 hydroxylates vitamin D2 at position C-25 position Homo sapiens 25-hydroxy-vitamin D2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.24 calciferol + O2 + [reduced NADPH-hemoprotein reductase] enzyme CYP2R1 hydroxylates vitamin D2 at position C-25 position Homo sapiens 25-hydroxyergocalciferol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.24 calciol + O2 + [reduced NADPH-hemoprotein reductase] enzyme CYP2R1 hydroxylates vitamin D3 at position C-25 position Homo sapiens calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.24 CYP2R1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.24 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.24 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.24 heme
-
 Homo sapiens
1.14.14.24 NADPH
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.14.24 physiological function CYP2R1 is a physiologically important vitamin D 25-hydroxylase. Vitamin D3 is initially converted to 25(OH)D3 in the liver, and then 25(OH)D 3 is converted to a functionally active form, namely 1alpha,25(OH)2D3, in the kidney. 1alpha,25(OH)2D3 plays essential roles in calcium and phosphate homeostasis, cell differentiation and immunology. Metabolic activation of vitamin D3 is performed by the hepatic vitamin D3 25-hydroxylase microsomal CYP2R1, and mitochondrial 1alpha-hydroxylase CYP27A1 Homo sapiens