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Literature summary extracted from

  • Patin, D.; Bayliss, M.; Mengin-Lecreulx, D.; Oyston, P.; Blanot, D.
    Purification and biochemical characterisation of GlmU from Yersinia pestis (2015), Arch. Microbiol., 197, 371-378.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.157 2-mercaptoethanol presence required Yersinia pestis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.157 expression in Escherichia coli Yersinia pestis
2.3.1.157 gene glmU, DNA and amino acid sequence determination, analysis, and comparison, functional complementation of the Escherichia coli glmU thermosensitive mutant strain UGS83, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Yersinia pseudotuberculosis
2.7.7.23 gene glmU, DNA and amino acid sequence determination, analysis, and comparison, functional complementation of the Escherichia coli glmU thermosensitive mutant strain UGS83, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Yersinia pseudotuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.157 C307S site-directed mutagenesis Yersinia pseudotuberculosis
2.7.7.23 C307S site-directed mutagenesis Yersinia pseudotuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.157 2-nitro-5-thiocyanatobenzoic acid 0.5 mM, 5% residual activity Yersinia pestis
2.3.1.157 5,5'-dithiobis-(2-nitrobenzoic acid) 0.1 mM, 33% residual activity Yersinia pestis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.157 0.3
-
 alpha-D-glucosamine 1-phosphate pH 8.2, 37°C Yersinia pestis
2.3.1.157 0.3
-
 alpha-D-glucosamine 1-phosphate pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis
2.3.1.157 0.52
-
 acetyl-CoA pH 8.2, 37°C Yersinia pestis
2.3.1.157 0.52
-
 acetyl-CoA pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis
2.7.7.23 0.061
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis
2.7.7.23 0.095
-
 UTP pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.157 Mg2+ required Yersinia pseudotuberculosis
2.3.1.157 Mg2+ required for both UDP-N-acetylglucosamine pyrophosphorylase and glucosamine-1-phosphate N-acetyltransferase activities, optimum concentration 5 mM Yersinia pestis
2.7.7.23 Mg2+ required Yersinia pseudotuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.157 52965
-
 MALDI-TOF MS, SDS-PAGE Yersinia pseudotuberculosis
2.7.7.23 52965
-
 MALDI-TOF MS, SDS-PAGE Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Yersinia pseudotuberculosis
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Yersinia pseudotuberculosis YPIII
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Yersinia pseudotuberculosis
-
 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Yersinia pseudotuberculosis YPIII
-
 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Yersinia pestis
-
-
-
2.3.1.157 Yersinia pestis YPIII
-
-
-
2.3.1.157 Yersinia pseudotuberculosis
-
-
-
2.3.1.157 Yersinia pseudotuberculosis YPIII
-
-
-
2.7.7.23 Yersinia pseudotuberculosis
-
-
-
2.7.7.23 Yersinia pseudotuberculosis YPIII
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.157 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Yersinia pseudotuberculosis
2.7.7.23 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Yersinia pseudotuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Yersinia pseudotuberculosis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Yersinia pestis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Yersinia pseudotuberculosis YPIII CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Yersinia pestis YPIII CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Yersinia pseudotuberculosis diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Yersinia pseudotuberculosis YPIII diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.157 ? x * 52965, recombinant His6-tagged enzyme, SDS-PAGE and mass spectrometry Yersinia pseudotuberculosis
2.3.1.157 More C324 does not form a disulphide bond with any other cysteine. This is consistent with the replacement of C307 by a serine in Y. pestis and the great distance between the three other cysteines (C296, C301 and C385) and C324 Yersinia pseudotuberculosis
2.7.7.23 ? x * 52965, recombinant His6-tagged enzyme, SDS-PAGE and mass spectrometry Yersinia pseudotuberculosis
2.7.7.23 More C324 does not form a disulphide bond with any other cysteine. This is consistent with the replacement of C307 by a serine in Y. pestis and the great distance between the three other cysteines (C296, C301 and C385) and C324 Yersinia pseudotuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.157 bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase
-
 Yersinia pestis
2.3.1.157 GlcN-1-P acetyltransferase
-
 Yersinia pseudotuberculosis
2.3.1.157 GlmU
-
 Yersinia pseudotuberculosis
2.3.1.157 GlmU
-
 Yersinia pestis
2.3.1.157 UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase
-
 Yersinia pseudotuberculosis
2.7.7.23 GlcNAc-1-P uridyltransferase
-
 Yersinia pseudotuberculosis
2.7.7.23 GlmU
-
 Yersinia pseudotuberculosis
2.7.7.23 UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase
-
 Yersinia pseudotuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.157 37
-
 assay at Yersinia pseudotuberculosis
2.7.7.23 37
-
 assay at Yersinia pseudotuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.157 828
-
 alpha-D-glucosamine 1-phosphate pH 8.2, 37°C Yersinia pestis
2.3.1.157 828
-
 alpha-D-glucosamine 1-phosphate pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis
2.3.1.157 853
-
 acetyl-CoA pH 8.2, 37°C Yersinia pestis
2.3.1.157 853
-
 acetyl-CoA pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis
2.7.7.23 0.75
-
 UTP pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis
2.7.7.23 0.95
-
 N-acetyl-alpha-D-glucosamine 1-phosphate pH 8.2, 37°C, recombinant His6-tagged enzyme Yersinia pseudotuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.157 8.2
-
 assay at Yersinia pseudotuberculosis
2.7.7.23 8.6
-
 assay at Yersinia pseudotuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.157 acetyl-CoA
-
 Yersinia pseudotuberculosis

General Information

EC Number General Information Comment Organism
2.3.1.157 evolution the GlmU proteins encoded by Yersinia pestis and Yersinia pseudotuberculosis are identical in amino acid sequence Yersinia pseudotuberculosis
2.3.1.157 physiological function the bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase enzyme GlmU is an essential gene in Yersinia Yersinia pseudotuberculosis
2.7.7.23 evolution the GlmU proteins encoded by Yersinia pestis and Yersinia pseudotuberculosis are identical in amino acid sequence Yersinia pseudotuberculosis
2.7.7.23 physiological function the bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase enzyme GlmU is an essential gene in Yersinia Yersinia pseudotuberculosis