Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kumar, G.; Guan, S.; Frantom, P.A.
    Biochemical characterization of the retaining glycosyltransferase glucosyl-3-phosphoglycerate synthase from Mycobacterium tuberculosis (2014), Arch. Biochem. Biophys., 564, 120-127.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.266 D134A substitution of a canonical GT-A D134XD136 aspartic acid, complete loss of activity Mycobacterium tuberculosis
2.4.1.266 D136A substitution of a canonical GT-A D134XD136 aspartic acid, complete loss of activity Mycobacterium tuberculosis
2.4.1.266 E232A kinetic parameters similar to wild-type Mycobacterium tuberculosis
2.4.1.266 H258A 25–40-fold increases in Km values Mycobacterium tuberculosis
2.4.1.266 R256A mutation in a flexible loop covering the active site, strong increase in Km values Mycobacterium tuberculosis
2.4.1.266 R261A kinetic parameters similar to wild-type Mycobacterium tuberculosis
2.4.1.266 T187A 100fold decrease in the value of kcat Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.266 3-phospho-D-glycerate competitive with UDP-glucose Mycobacterium tuberculosis
2.4.1.266 UMP competitive with UDP-glucose, uncompetitive with 3-phospho-D-glycerate Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.266 0.2
-
 3-phospho-D-glycerate pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.25
-
 3-phospho-D-glycerate mutant E232A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.48
-
 UDP-glucose mutant T187A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.58
-
 UDP-glucose mutant E232A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.74
-
 3-phospho-D-glycerate mutant T187A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.82
-
 UDP-glucose wild-type, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 1.1
-
 3-phospho-D-glycerate mutant R261A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 1.5
-
 UDP-glucose mutant R261A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 8
-
 3-phospho-D-glycerate mutant H258A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 20
-
 UDP-glucose mutant H258A, pH 7.8, 25°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.266 Mycobacterium tuberculosis P9WMW9
-
-
2.4.1.266 Mycobacterium tuberculosis ATCC 25618 P9WMW9
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.266 NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate ordered kinetic mechanism with 3-phosphoglycerate binding first, followed by UDP-glucose Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.266 additional information a single ionizable residue is involved catalysis (pKa = 6.3) that must be deprotonated for full activity. A solvent kinetic isotope effect of 2.0 on kcat is consistent with a proton in flight during the rate-determining step Mycobacterium tuberculosis ?
-
 ?
2.4.1.266 additional information a single ionizable residue is involved catalysis (pKa = 6.3) that must be deprotonated for full activity. A solvent kinetic isotope effect of 2.0 on kcat is consistent with a proton in flight during the rate-determining step Mycobacterium tuberculosis ATCC 25618 ?
-
 ?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
 Mycobacterium tuberculosis UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
 Mycobacterium tuberculosis ATCC 25618 UDP + 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.266 0.012
-
 UDP-glucose mutant T187A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.38
-
 UDP-glucose mutant R261A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.75
-
 UDP-glucose mutant H258A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.82
-
 UDP-glucose wild-type, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.83
-
 UDP-glucose mutant E232A, pH 7.8, 25°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.266 0.21
-
 UMP substrate UDP-glucose, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.22
-
 UMP substrate 3-phospho-D-glycerate, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 21
-
 3-phospho-D-glycerate substrate UDP-glucose, pH 7.8, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.266 0.014
-
 3-phospho-D-glycerate mutant T187A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.024
-
 UDP-glucose mutant T187A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.034
-
 3-phospho-D-glycerate mutant H258A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.038
-
 UDP-glucose mutant H258A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.248
-
 UDP-glucose mutant R261A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 0.31
-
 3-phospho-D-glycerate mutant R261A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 1.12
-
 UDP-glucose wild-type, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 1.28
-
 UDP-glucose mutant E232A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 3.42
-
 3-phospho-D-glycerate mutant E232A, pH 7.8, 25°C Mycobacterium tuberculosis
2.4.1.266 3.58
-
 3-phospho-D-glycerate pH 7.8, 25°C Mycobacterium tuberculosis