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Literature summary extracted from

  • Wielgus-Kutrowska, B.; Modrak-Wojcik, A.; Dyzma, A.; Breer, K.; Zolkiewski, M.; Bzowska, A.
    Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme (2014), Arch. Biochem. Biophys., 549, 40-48.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expressed in Escherichia coli BL21(DE3) cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.1 F159W the mutant shows reduced activity and stability compared to the wild type enzyme Bos taurus
2.4.2.1 F200W the mutant shows reduced activity and stability compared to the wild type enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 31654
-
 3 * 31654, calculated from amino acid sequence Bos taurus
2.4.2.1 32000
-
 3 * 32000, SDS-PAGE Bos taurus
2.4.2.1 100000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.1 purine deoxyribonucleoside + phosphate Bos taurus
-
 purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 purine ribonucleoside + phosphate Bos taurus
-
 purine + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Bos taurus P55859
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 spleen
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.4.2.1 25°C, phosphate buffer pH 7.0, 130 h, 30% loss of activity Bos taurus
2.4.2.1 38°C, phosphate buffer pH 7.0, 130 h, 24% loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate
-
 Bos taurus adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Bos taurus hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 purine deoxyribonucleoside + phosphate
-
 Bos taurus purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 purine ribonucleoside + phosphate
-
 Bos taurus purine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 homotrimer 3 * 32000, SDS-PAGE Bos taurus
2.4.2.1 homotrimer 3 * 31654, calculated from amino acid sequence Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 PNP
-
 Bos taurus
2.4.2.1 purine nucleoside phosphorylase
-
 Bos taurus