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Literature summary extracted from

  • Barney, B.M.; Ohlert, J.M.; Timler, J.G.; Lijewski, A.M.
    Altering small and medium alcohol selectivity in the wax ester synthase (2015), Appl. Microbiol. Biotechnol., 99, 9675-9684.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.75 L356A mutation increases selectivity toward ethanol Marinobacter nauticus
2.3.1.75 L356F mutation increases selectivity toward ethanol, with a drop in specific activity of approximately 89% for dodecanol Marinobacter nauticus
2.3.1.75 L356V mutation increases selectivity toward 2-phenylethanol Marinobacter nauticus
2.3.1.75 L356Y substantial loss of activity Marinobacter nauticus
2.3.1.75 M405A significant decrease in overall activity with dodecanol Marinobacter nauticus
2.3.1.75 M405F mutation increases selectivity toward isoamyl alcohol, significant decrease in overall activity with dodecanol Marinobacter nauticus
2.3.1.75 M405L significant decrease in overall activity with dodecanol Marinobacter nauticus
2.3.1.75 M405W dramatically shifts selectivity toward ethanol, significant decrease in overall activity with dodecanol Marinobacter nauticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.75 ethanol substrate inhibition above 0.5 M Marinobacter nauticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.75 2.4
-
 hexan-1-ol wild-type, temperature not specified in the publication, pH not specified in the publication Marinobacter nauticus
2.3.1.75 5.6
-
 isoamyl alcohol wild-type, temperature not specified in the publication, pH not specified in the publication Marinobacter nauticus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.75 Marinobacter nauticus A1TX06
-
-
2.3.1.75 Marinobacter nauticus DSM 11845 A1TX06
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.75 2-phenylethanol + palmitoyl-CoA
-
 Marinobacter nauticus 2-phenylethyl palmitate + CoA
-
 ?
2.3.1.75 2-phenylethanol + palmitoyl-CoA
-
 Marinobacter nauticus DSM 11845 2-phenylethyl palmitate + CoA
-
 ?
2.3.1.75 ethanol + palmitoyl-CoA
-
 Marinobacter nauticus ethyl palmitate + CoA
-
 ?
2.3.1.75 ethanol + palmitoyl-CoA
-
 Marinobacter nauticus DSM 11845 ethyl palmitate + CoA
-
 ?
2.3.1.75 hexan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus hexyl palmitate + CoA
-
 ?
2.3.1.75 hexan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus DSM 11845 hexyl palmitate + CoA
-
 ?
2.3.1.75 isoamyl alcohol + palmitoyl-CoA
-
 Marinobacter nauticus isoamyl palmitate + CoA
-
 ?
2.3.1.75 isoamyl alcohol + palmitoyl-CoA
-
 Marinobacter nauticus DSM 11845 isoamyl palmitate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.75 Maqu_0168
-
 Marinobacter nauticus