EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.231 | DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli, functional coexpression of plant enzymes, e.g. 4-coumaroyl-CoA ligase and chalcone synthase, of different origins catalyzing steps in flavonoid biosynthesis pathways for production of naringenin from p-coumaric acid and synthesis of ponciretin and sakuranetin from naringenin in engineered and transformed Escherichia coli strain BL21(DE3) | Glycine max |
2.1.1.232 | gene Os12g0240900, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli, functional coexpression of plant enzymes, e.g. 4-coumaroyl-CoA ligase and chalcone synthase, of different origins catalyzing steps in flavonoid biosynthesis pathways for production of naringenin from p-coumaric acid and synthesis of ponciretin and sakuranetin from naringenin in engineered and transformed Escherichia coli strain BL21(DE3) | Oryza sativa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.231 | S-adenosyl-L-methionine + naringenin | Glycine max | i.e. 4',5,7-trihyroxyflavanone, the enzyme transfers a methyl group to 4'-hydroxyl group of naringenin | S-adenosyl-L-homocysteine + ponciretin | i.e. 4'-methoxy-5,7-dihydroxyflavanone | ? | |
2.1.1.232 | S-adenosyl-L-methionine + (2S)-naringenin | Oryza sativa | reaction of NOMT | S-adenosyl-L-homocysteine + (2S)-sakuranetin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.231 | Glycine max | C6TAY1 | cv. Paldal | - |
2.1.1.232 | Oryza sativa | Q0IP69 | cv. Nakdong, gene NOMT-2 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.231 | S-adenosyl-L-methionine + naringenin | i.e. 4',5,7-trihyroxyflavanone, the enzyme transfers a methyl group to 4'-hydroxyl group of naringenin | Glycine max | S-adenosyl-L-homocysteine + ponciretin | i.e. 4'-methoxy-5,7-dihydroxyflavanone | ? | |
2.1.1.231 | S-adenosyl-L-methionine + naringenin | i.e. 4',5,7-trihyroxyflavanone, reaction of SOMT-2 | Glycine max | S-adenosyl-L-homocysteine + ponciretin | i.e. 4'-methoxy-5,7-dihydroxyflavanone | ? | |
2.1.1.232 | S-adenosyl-L-methionine + (2S)-naringenin | reaction of NOMT | Oryza sativa | S-adenosyl-L-homocysteine + (2S)-sakuranetin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.231 | SOMT2 | - |
Glycine max |
2.1.1.231 | soybean O-methyltransferase | - |
Glycine max |
2.1.1.232 | naringenin O-methyltransferase | - |
Oryza sativa |
2.1.1.232 | NOMT | - |
Oryza sativa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.231 | S-adenosyl-L-methionine | - |
Glycine max | |
2.1.1.232 | S-adenosyl-L-methionine | - |
Oryza sativa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.231 | metabolism | the enzyme catalyzes the last step in the biosynthesis of the bioactive flavonoid ponciretin, biosynthetic pathways starting with glucose, overview | Glycine max |
2.1.1.232 | metabolism | the enzyme catalyzes the last step in the biosynthesis of the bioactive flavonoid sakuranetin, biosynthetic pathway starting with glucose, overview | Oryza sativa |