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Literature summary extracted from

  • Ochi, A.; Matsumoto, K.; Ooba, T.; Sakai, K.; Tsuge, T.; Taguchi, S.
    Engineering of class i lactate-polymerizing polyhydroxyalkanoate synthases from Ralstonia eutropha that synthesize lactate-based polyester with a block nature (2013), Appl. Microbiol. Biotechnol., 97, 3441-3447.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.304 synthesis mutant A510S is able to synthesize a lactate-3-hydroxybutanoate copolymer containing 7 mol% lactate and with a averge molecular weight of 320000 Da. The polymer contains a high ratio of an LA-LA-LA triad sequence Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 A510S mutant is able to synthesize a lactate-3-hydroxybutanoate copolymer containing 7 mol% lactate and with a averge molecular weight of 320000 Da. The polymer contains a high ratio of an LA-LA-LA triad sequence Cupriavidus necator
2.3.1.304 A510X mutation corresponds to position 481 in the class II lactate polymerizing polyhydroxyalkanoate synthase PhaC1PsSTQK, in which Gln481Lys is essential to its lactate polymerizing activity. Among 19 A510X mutants, 15 synthesize lactate-3-hydroxybutanoate copolymers Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Cupriavidus necator P23608
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2.3.1.304 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 P23608
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Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 PhaC
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Cupriavidus necator