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Literature summary extracted from

  • Kayser, K.J.; Cleveland, L.; Park, H.S.; Kwak, J.H.; Kolhatkar, A.; Kilbane, J.J.
    Isolation and characterization of a moderate thermophile, Mycobacterium phlei GTIS10, capable of dibenzothiophene desulfurization (2002), Appl. Microbiol. Biotechnol., 59, 737-745.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.21 gene dszC, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha Mycolicibacterium phlei
1.14.14.22 gene dszA, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Mycolicibacterium phlei
-
 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2 Mycolicibacterium phlei GTIS10
-
 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 additional information Mycolicibacterium phlei the dszC gene encodes DBT monooxygenase, which catalyzes the conversion of dibenzothiophene to dibenzothiophene sulfone ?
-
 ?
1.14.14.21 additional information Mycolicibacterium phlei GTIS10 the dszC gene encodes DBT monooxygenase, which catalyzes the conversion of dibenzothiophene to dibenzothiophene sulfone ?
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Mycolicibacterium phlei
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Mycolicibacterium phlei GTIS10
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 additional information Mycolicibacterium phlei The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate ?
-
 ?
1.14.14.22 additional information Mycolicibacterium phlei GTIS10 The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.21 Mycolicibacterium phlei
-
 gene dszC
-
1.14.14.21 Mycolicibacterium phlei GTIS10
-
 gene dszC
-
1.14.14.22 Mycolicibacterium phlei
-
-
-
1.14.14.22 Mycolicibacterium phlei GTIS10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.21 recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinty chromatography Mycolicibacterium phlei
1.14.14.22 recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinty chromatography Mycolicibacterium phlei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.21 cell culture the organism can use dibenzothiophene as a sole source of sulfur for growth at 30-52°C, it can also utilize benzothiophene and thiophene as sulfur sources for growth. Range of organosulfur substrates utilized as a sole source of sulfur for growth by Mycobacterium phlei GTIS10, best is dibenzothiophene, overview Mycolicibacterium phlei
-
1.14.14.22 cell culture the organism can use dibenzothiophene as a sole source of sulfur for growth at 30-52°C, it can also utilize benzothiophene and thiophene as sulfur sources for growth. Range of organosulfur substrates utilized as a sole source of sulfur for growth by Mycobacterium phlei GTIS10, best is dibenzothiophene, overview Mycolicibacterium phlei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
 Mycolicibacterium phlei dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 dibenzothiophene + 2 FMNH2 + 2 O2
-
 Mycolicibacterium phlei GTIS10 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
1.14.14.21 additional information the dszC gene encodes DBT monooxygenase, which catalyzes the conversion of dibenzothiophene to dibenzothiophene sulfone Mycolicibacterium phlei ?
-
 ?
1.14.14.21 additional information the dszC gene encodes DBT monooxygenase, which catalyzes the conversion of dibenzothiophene to dibenzothiophene sulfone Mycolicibacterium phlei GTIS10 ?
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Mycolicibacterium phlei 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Mycolicibacterium phlei GTIS10 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
1.14.14.22 additional information The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate Mycolicibacterium phlei ?
-
 ?
1.14.14.22 additional information The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate Mycolicibacterium phlei GTIS10 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.21 DBT monooxygenase
-
 Mycolicibacterium phlei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.21 50
-
 assay at Mycolicibacterium phlei
1.14.14.22 50
-
 assay at Mycolicibacterium phlei

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.21 FMNH2
-
 Mycolicibacterium phlei
1.14.14.22 FMNH2
-
 Mycolicibacterium phlei

General Information

EC Number General Information Comment Organism
1.14.14.21 physiological function Mycobacterium phlei strain GTIS10 converts dibenzothiophene to 2'-hydroxybiphenyl, determination of metabolites from dibenzothiophene, overview Mycolicibacterium phlei
1.14.14.22 physiological function Mycobacterium phlei strain GTIS10 converts dibenzothiophene to 2'-hydroxybiphenyl, determination of metabolites from dibenzothiophene, overview Mycolicibacterium phlei