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Literature summary extracted from

  • Ohashi, T.; Hasegawa, Y.; Misaki, R.; Fujiyama, K.
    Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast (2016), Appl. Microbiol. Biotechnol., 100, 687-696.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.B53 synthesis naringin and narirutin are produced from naringenin-7-O-glucoside using engineered fission yeast expressing the Arabidopsis thaliana rhamnose synthase RHM2 and the Citrus maxima alpha1,2-rhamnosyltransferase or the Citrus sinensis alpha16rhamnosyl transferase genes as a whole-cell-biocatalyst Citrus sinensis
2.4.1.B53 synthesis naringin and narirutin are produced from naringenin-7-O-glucoside using engineered fission yeast expressing the Arabidopsis thaliana rhamnose synthase RHM2 and the Citrus maxima alpha1,2-rhamnosyltransferase or the Citrus sinensis alpha16rhamnosyl transferase genes as a whole-cell-biocatalyst Citrus maxima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.B53 expression in Schizosaccharomyces pombe Citrus sinensis
2.4.1.B53 expression in Schizosaccharomyces pombe Citrus maxima
2.4.1.236 DNA and amino acid sequence determiation and analysis, phylogenetic analysis, recombinant expression of C-terminally His6-Myc-tagged enzyme in Nicotiana tabacum BY-2 cells Citrus maxima

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.236 additional information production of naringin and narirutin from naringenin-7-O-glucoside using the engineered Schizosaccharomyces expressing Arabidopsis thaliana rhamnose synthase 2, AtRHM2, and glycosyltransferases Cm1,2RhaT from Citrus maxima or Cs1,6RhaT from Citrus sinensis as a whole-cell-biocatalyst, overview Citrus maxima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.B53 54000
-
 x * 54000, SDS-PAGE Citrus maxima
2.4.1.236 52000
-
 x * 52000, recombinant C-terminally His6-Myc-tagged enzyme, SDS-PAGE Citrus maxima

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B53 Citrus maxima A0A0P0YNP7
-
-
2.4.1.B53 Citrus sinensis A7ISD3
-
-
2.4.1.236 Citrus maxima A0A0P0YNP7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B53 additional information no substrates: UDP-Xyl, UDP-Glc Citrus sinensis ?
-
 ?
2.4.1.B53 additional information no substrates: UDP-Xyl, UDP-Glc Citrus maxima ?
-
 ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside
-
 Citrus sinensis UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + naringenin 7-O-beta-D-glucoside
-
 Citrus maxima UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 3-O-beta-D-glucoside
-
 Citrus sinensis UDP + quercetin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.B53 UDP-L-rhamnose + quercetin 3-O-beta-D-glucoside
-
 Citrus maxima UDP + quercetin 3-O-[alpha-L-rhamnosyl-(1->6)-beta-D-glucoside]
-
 ?
2.4.1.236 additional information Cm1,2RhaT has glycosyl transfer activity toward UDP-xylose as well as UDP-rhamnose, it is a branch-forming rhamnosyltransferase, substrate specificity of the recombinant enzyme, overview. No activity with quercetin-3-O-glucoside Citrus maxima ?
-
 ?
2.4.1.236 UDP-beta-L-rhamnose + a flavanone 7-O-beta-D-glucoside
-
 Citrus maxima UDP + a flavanone 7-O-[alpha-L-rhamnosyl-(1->2)-beta-D-glucoside]
-
 ?
2.4.1.236 UDP-beta-L-rhamnose + naringenin-7-O-glucoside
-
 Citrus maxima UDP + naringenin 7-O-[alpha-L-rhamnosyl-(1->2)-beta-D-glucoside]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.B53 ? x * 54000, SDS-PAGE Citrus maxima
2.4.1.236 ? x * 52000, recombinant C-terminally His6-Myc-tagged enzyme, SDS-PAGE Citrus maxima

Synonyms

EC Number Synonyms Comment Organism
2.4.1.236 Cm1,2RhaT
-
 Citrus maxima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.236 30
-
 assay at Citrus maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.236 7.5
-
 assay at Citrus maxima