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Literature summary extracted from

  • Wu, H.; Li, W.; Xin, C.; Zhang, C.; Wang, Y.; Ren, S.; Ren, M.; Zhao, W.; Yuan, L.; Xu, Z.; Yuan, H.; Geng, M.; Zhang, L.; Weaver, D.T.; Zhang, B.
    In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea (2016), Appl. Microbiol. Biotechnol., 100, 2257-2266.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.277 Streptomyces venezuelae Q9ZGH7
-
-
2.4.1.278 Streptomyces venezuelae Q9ZGH7 enzyme catalyzes reaction of EC 2.4.1.277
-
2.4.1.328 Streptomyces venezuelae Q9ZGH7 enzyme catalyzes reaction of EC 2.4.1.277
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.277 dTDP-beta-L-mycarose + erythronolide B
-
 Streptomyces venezuelae dTDP + 3-alpha-L-mycarosylerythronolide B reaction of EC 2.4.1.328 ?
2.4.1.278 dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B
-
 Streptomyces venezuelae dTDP + erythromycin D
-
 ?
2.4.1.328 dTDP-beta-L-mycarose + erythronolide B
-
 Streptomyces venezuelae dTDP + 3-alpha-L-mycarosylerythronolide B
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.277 DesVII
-
 Streptomyces venezuelae
2.4.1.278 DesVII
-
 Streptomyces venezuelae
2.4.1.328 DesVII
-
 Streptomyces venezuelae

General Information

EC Number General Information Comment Organism
2.4.1.277 physiological function coexpression of glycosyltransferase DesVII and its auxiliary partner DesVIII in a Saccharopolyspora erythraea EryBV deletion mutant leads to an increased erythromycin A yield by 15% in comparison to Saccharopolyspora erythraea wild-type. DesVII/DesVIII replaced EryCIII to upload D-desosamine to C5 position of 3-O-mycarosyl erythronolide B and also in vivo attache L-mycarose, not D-desosamine to C3 position of erythronolide B with a higher activity than EryBV. Expression of DesVII in EryCIII and EryBV-CIII mutant strains partially restores the erythromycin A production, however, no erythromycin A is detected while DesVII is expressed in the EryBV mutant strain Streptomyces venezuelae
2.4.1.278 physiological function coexpression of glycosyltransferase DesVII and its auxiliary partner DesVIII in a Saccharopolyspora erythraea EryBV deletion mutant leads to an increased erythromycin A yield by 15% in comparison to Saccharopolyspora erythraea wild-type. DesVII/DesVIII replaced EryCIII to upload D-desosamine to C5 position of 3-O-mycarosyl erythronolide B and also in vivo attache L-mycarose, not D-desosamine to C3 position of erythronolide B with a higher activity than EryBV. Expression of DesVII in EryCIII and EryBV-CIII mutant strains partially restores the erythromycin A production, however, no erythromycin A is detected while DesVII is expressed in the EryBV mutant strain Streptomyces venezuelae
2.4.1.328 physiological function coexpression of glycosyltransferase DesVII and its auxiliary partner DesVIII in a Saccharopolyspora erythraea EryBV deletion mutant leads to an increased erythromycin A yield by 15% in comparison to Saccharopolyspora erythraea wild-type. DesVII/DesVIII replaced EryCIII to upload D-desosamine to C5 position of 3-O-mycarosyl erythronolide B and also in vivo attache L-mycarose, not D-desosamine to C3 position of erythronolide B with a higher activity than EryBV. Expression of DesVII in EryCIII and EryBV-CIII mutant strains partially restores the erythromycin A production, however, no erythromycin A is detected while DesVII is expressed in the EryBV mutant strain Streptomyces venezuelae