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Literature summary extracted from

  • Hobdey, S.E.; Knott, B.C.; Haddad Momeni, M.; Taylor, L.E.; Borisova, A.S.; Podkaminer, K.K.; VanderWall, T.A.; Himmel, M.E.; Decker, S.R.; Beckham, G.T.; Stahlberg, J.
    Biochemical and structural characterization of two Dictyostelium cellobiohydrolases from the Amoebozoa kingdom reveal a high conservation between distant phylogenetic trees of life (2016), Appl. Environ. Microbiol., 82, 3395-3409.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.176 to 1.8 A resolution. Protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Structure is homologous to those of other GH7 CBHs with an enclosed active-site tunnel Dictyostelium purpureum
3.2.1.176 to 2.1 A resolution. Protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Strucutre is homologous to those of other GH7 CBHs with an enclosed active-site tunnel Dictyostelium discoideum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.176 additional information protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Upon addition of the family 1 Hypocrea jecorina Cel7A carbohydrate-binding module and linker to the C terminus of the Dictyostelium enzyme, the recombinant Cel7ACBM hydrolyzes avicel, pretreated corn stover, and phosphoric acid-swollen cellulose as efficiently as Hypocrea jecorina Cel7A when hydrolysis is compared at their temperature optima. The Ki of cellobiose is significantly higher for the Dictyostelium Cel7ACBM Dictyostelium purpureum
3.2.1.176 additional information protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Upon addition of the family 1 Hypocrea jecorina Cel7A carbohydrate-binding module and linker to the C terminus of the Dictyostelium enzymes, the recombinant Cel7ACBM hydrolyzes avicel, pretreated corn stover, and phosphoric acid-swollen cellulose as efficiently as Hypocrea jecorina Cel7A when hydrolysis is compared at their temperature optima. The Ki of cellobiose is significantly higher for the Dictyostelium Cel7ACBM Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose
-
 Dictyostelium discoideum
3.2.1.176 cellobiose
-
 Dictyostelium purpureum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 3.4
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
3.2.1.176 3.4
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Dictyostelium discoideum Q55FE6
-
-
3.2.1.176 Dictyostelium purpureum F0ZJZ1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-nitrophenyl beta-D-lactoside + H2O
-
 Dictyostelium discoideum 4-nitrophenol + lactose
-
 ?
3.2.1.176 4-nitrophenyl beta-D-lactoside + H2O
-
 Dictyostelium purpureum 4-nitrophenol + lactose
-
 ?
3.2.1.176 avicel + H2O
-
 Dictyostelium discoideum ?
-
 ?
3.2.1.176 avicel + H2O
-
 Dictyostelium purpureum ?
-
 ?
3.2.1.176 phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium discoideum ?
-
 ?
3.2.1.176 phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium purpureum ?
-
 ?
3.2.1.176 pretreated corn stover + H2O
-
 Dictyostelium discoideum ?
-
 ?
3.2.1.176 pretreated corn stover + H2O
-
 Dictyostelium purpureum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 Cel7A
-
 Dictyostelium discoideum
3.2.1.176 Cel7A
-
 Dictyostelium purpureum
3.2.1.176 DDB0202233
-
 Dictyostelium discoideum
3.2.1.176 DICPUDRAFT_151874
-
 Dictyostelium purpureum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 45
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
3.2.1.176 55
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.176 53
-
 melting temperature, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
3.2.1.176 53
-
 melting temperature, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.176 0.27
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
3.2.1.176 0.51
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 5
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
3.2.1.176 5
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.176 0.13
-
 cellobiose pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum
3.2.1.176 0.295
-
 cellobiose pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum