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Literature summary extracted from

  • Kasai, D.; Araki, N.; Motoi, K.; Yoshikawa, S.; Iino, T.; Imai, S.; Masai, E.; Fukuda, M.
    gamma-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1 (2015), Appl. Environ. Microbiol., 81, 7656-7665.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32 expressed in Escherichia coli BL21(DE3) cells Rhodococcus jostii
1.14.13.220 expression in Escherichia coli BL21 Rhodococcus jostii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 34000
-
 x * 34000, SDS-PAGE Rhodococcus jostii
1.14.13.220 59000
-
 1 * 59000, SDS-PAGE Rhodococcus jostii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADH + H+ Rhodococcus jostii
-
 3-oxoadipate + NAD+
-
 ?
1.3.1.32 2-maleylacetate + NADPH + H+ Rhodococcus jostii
-
 3-oxoadipate + NADP+
-
 ?
1.14.13.220 resorcinol + NADH + H+ + O2 Rhodococcus jostii the enzyme gamma-resorcylate catabolic-pathway hydroxyquinol + NAD+ + H2O
-
 ?
4.1.1.103 2,6-dihydroxybenzoate Rhodococcus jostii
-
 1,3-dihydroxybenzene + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Rhodococcus jostii
-
-
-
1.14.13.220 Rhodococcus jostii
-
-
-
4.1.1.103 Rhodococcus jostii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.220 recombinant His-tagged protein Rhodococcus jostii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-maleylacetate + NADH + H+
-
 Rhodococcus jostii 3-oxoadipate + NAD+
-
 ?
1.3.1.32 2-maleylacetate + NADPH + H+
-
 Rhodococcus jostii 3-oxoadipate + NADP+
-
 ?
1.14.13.220 resorcinol + NADH + H+ + O2 the enzyme gamma-resorcylate catabolic-pathway Rhodococcus jostii hydroxyquinol + NAD+ + H2O
-
 ?
1.14.13.220 resorcinol + NADH + H+ + O2 resorcinol i.e. benzene-1,3-diol. The enzyme has no activity with NADPH Rhodococcus jostii hydroxyquinol + NAD+ + H2O hydroxyquinol i.e. benzene-1,2,4-triol ?
4.1.1.103 2,6-dihydroxybenzoate
-
 Rhodococcus jostii 1,3-dihydroxybenzene + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.32 ? x * 34000, SDS-PAGE Rhodococcus jostii
1.14.13.220 monomer 1 * 59000, SDS-PAGE Rhodococcus jostii

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 tsdD
-
 Rhodococcus jostii
1.14.13.220 tsdB
-
 Rhodococcus jostii
4.1.1.103 TsdA
-
 Rhodococcus jostii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.220 30
-
 assay at Rhodococcus jostii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.220 8
-
 assay at Rhodococcus jostii

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Rhodococcus jostii
1.3.1.32 NADPH
-
 Rhodococcus jostii
1.14.13.220 NADH the enzyme has no activity with NADPH Rhodococcus jostii

Expression

EC Number Organism Comment Expression
1.14.13.220 Rhodococcus jostii transcription of tsdB is activated when cells are grown on gamma-resorcylate up

General Information

EC Number General Information Comment Organism
1.14.13.220 metabolism the enzyme gamma-resorcylate catabolic-pathway Rhodococcus jostii
4.1.1.103 physiological function expression of the tsdA gene confers gamma-resorcylate decarboxylase activity on Escherichia coli. Transcription of the tsdBADC and tsdTX operons required for gamma-resorcylate catabolism is induced during growth on gamma-resorcylate. Inactivation of tsdR derepresses transcription of the tsdBADC and tsdTX operons in the absence of gamma-resorcylate Rhodococcus jostii