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Literature summary extracted from

  • Barney, B.; Mann, R.; Ohlert, J.
    Identification of a residue affecting fatty alcohol selectivity in wax ester synthase (2013), Appl. Environ. Microbiol., 79, 396-399.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.75
-
 Acinetobacter baylyi
2.3.1.75
-
 Marinobacter nauticus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.75 A360F mutation slightly improves the rate of reaction found for smaller fatty alcohols Marinobacter nauticus
2.3.1.75 A360I mutation improves the rate of reaction found for smaller fatty alcohols Marinobacter nauticus
2.3.1.75 A360V mutation improves the rate of reaction found for smaller fatty alcohols Marinobacter nauticus
2.3.1.75 G355I mutation improves the rate of reaction Acinetobacter baylyi

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.75 Acinetobacter baylyi Q8GGG1
-
-
2.3.1.75 Marinobacter nauticus A1TX06
-
-
2.3.1.75 Marinobacter nauticus DSM 11845 A1TX06
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.75 1.6
-
 substrate hexadecan-1-ol, mutant A360I, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 1.64
-
 substrate hexadecan-1-ol, mutant A360V, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 1.85
-
 substrate hexadecan-1-ol, mutant A360F, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 2.7
-
 substrate hexadecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 3.6
-
 substrate nonan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 7.8
-
 substrate nonan-1-ol, mutant A360F, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 16.6
-
 substrate nonan-1-ol, mutant A360V, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 19.6
-
 substrate nonan-1-ol, mutant A360I, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 34.3
-
 substrate dodecan-1-ol, mutant A360I, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 34.6
-
 substrate dodecan-1-ol, mutant A360F, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 35.9
-
 substrate dodecan-1-ol, mutant A360V, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 46.5
-
 substrate dodecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Marinobacter nauticus
2.3.1.75 70
-
 substrate hexadecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
2.3.1.75 80
-
 substrate hexadecan-1-ol, mutant G355I, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
2.3.1.75 140
-
 substrate nonan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
2.3.1.75 360
-
 substrate nonan-1-ol, mutant G355I, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
2.3.1.75 960
-
 substrate dodecan-1-ol, wild-type, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi
2.3.1.75 1700
-
 substrate dodecan-1-ol, mutant G355I, pH not specified in the publication, temperature not specified in the publication Acinetobacter baylyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.75 dodecan-1-ol + palmitoyl-CoA
-
 Acinetobacter baylyi CoA + dodecanyl palmitate
-
 ?
2.3.1.75 dodecan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus CoA + dodecanyl palmitate
-
 ?
2.3.1.75 dodecan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus DSM 11845 CoA + dodecanyl palmitate
-
 ?
2.3.1.75 hexadecan-1-ol + palmitoyl-CoA
-
 Acinetobacter baylyi CoA + hexadecyl palmitate
-
 ?
2.3.1.75 hexadecan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus CoA + hexadecyl palmitate
-
 ?
2.3.1.75 hexadecan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus DSM 11845 CoA + hexadecyl palmitate
-
 ?
2.3.1.75 nonan-1-ol + palmitoyl-CoA
-
 Acinetobacter baylyi CoA + nonyl palmitate
-
 ?
2.3.1.75 nonan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus CoA + nonyl palmitate
-
 ?
2.3.1.75 nonan-1-ol + palmitoyl-CoA
-
 Marinobacter nauticus DSM 11845 CoA + nonyl palmitate
-
 ?