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Literature summary extracted from

  • Liu, K.; Liu, T.T.; Zhou, N.Y.
    HbzF catalyzes direct hydrolysis of maleylpyruvate in the gentisate pathway of Pseudomonas alcaligenes NCIMB 9867 (2013), Appl. Environ. Microbiol., 79, 1044-1047.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.23 expressed in Escherichia coli BL21(DE3) cells Pseudomonas alcaligenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.23 Cd2+ complete inhibition at 1 mM Pseudomonas alcaligenes
3.7.1.23 Co2+ complete inhibition at 1 mM Pseudomonas alcaligenes
3.7.1.23 Cu2+ complete inhibition at 1 mM Pseudomonas alcaligenes
3.7.1.23 Ni2+ complete inhibition at 1 mM Pseudomonas alcaligenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.23 0.0199
-
 3-maleylpyruvate at pH 7.6 and 30°C Pseudomonas alcaligenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.7.1.23 Mn2+ addition of Mn2+ at 0.05 or 0.1 mM enhances the enzyme activity by 30% Pseudomonas alcaligenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.23 33000
-
 2 * 33000, SDS-PAGE Pseudomonas alcaligenes
3.7.1.23 77000
-
 gel filtration Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.23 3-maleylpyruvate + H2O Pseudomonas alcaligenes
-
 maleate + pyruvate
-
 ?
3.7.1.23 3-maleylpyruvate + H2O Pseudomonas alcaligenes NCIMB 9867
-
 maleate + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.23 Pseudomonas alcaligenes Q0QFQ3
-
-
3.7.1.23 Pseudomonas alcaligenes NCIMB 9867 Q0QFQ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.23 Ni Sepharose column chromatography Pseudomonas alcaligenes

Storage Stability

EC Number Storage Stability Organism
3.7.1.23 -80°C, with 50% (v/v) glycerol, 20 days, 38% loss of activity Pseudomonas alcaligenes
3.7.1.23 -80°C, with 50% (v/v) glycerol, 50 days, 73% loss of activity Pseudomonas alcaligenes
3.7.1.23 4°C, with 5% (v/v) glycerol, 10 days, 50% loss of activity Pseudomonas alcaligenes
3.7.1.23 4°C, with 5% (v/v) glycerol, 40 days, 79% loss of activity Pseudomonas alcaligenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.23 3-maleylpyruvate + H2O
-
 Pseudomonas alcaligenes maleate + pyruvate
-
 ?
3.7.1.23 3-maleylpyruvate + H2O
-
 Pseudomonas alcaligenes NCIMB 9867 maleate + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.23 homodimer 2 * 33000, SDS-PAGE Pseudomonas alcaligenes

Synonyms

EC Number Synonyms Comment Organism
3.7.1.23 hbzF
-
 Pseudomonas alcaligenes
3.7.1.23 maleylpyruvate hydrolase
-
 Pseudomonas alcaligenes
3.7.1.23 Mph
-
 Pseudomonas alcaligenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.7.1.23 30
-
-
 Pseudomonas alcaligenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.23 7.6
-
-
 Pseudomonas alcaligenes