EC Number | General Stability | Organism |
---|---|---|
1.1.1.391 | EDTA has no effect enzyme thermal stability | [Clostridium] innocuum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.391 | 3-oxo bile acids | the specific enzyme activity is significantly reduced by growth medium supplementation with 3-oxo bile acids, with trisubstituted acids being more effective than disubstituted ones | [Clostridium] innocuum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.391 | additional information | - |
additional information | Michaelis-Menten kinetis | [Clostridium] innocuum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.391 | membrane | membrane-bound | [Clostridium] innocuum | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.391 | 56000 | - |
gel filtration | [Clostridium] innocuum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.391 | 3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum | - |
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum D1144/3 | - |
3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum | - |
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | [Clostridium] innocuum D1144/3 | - |
3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.391 | [Clostridium] innocuum | - |
- |
- |
1.1.1.391 | [Clostridium] innocuum D1144/3 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.391 | native enzyme 6.3fold by anion exchange chromatography and gel filtration | [Clostridium] innocuum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.391 | additional information | the enzyme production is constitutive in the absence of added bile acids | [Clostridium] innocuum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.391 | 3.2 | - |
purified enzyme, pH 10.0-10.2, 37°C | [Clostridium] innocuum |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.391 | 4°C, crude enzyme extract, pH 8.5, in the presence of dithiothreitol, no loss of activity after 24 h | [Clostridium] innocuum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.391 | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | sole pruduct | ? | |
1.1.1.391 | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | sole pruduct | ? | |
1.1.1.391 | 3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 3,12-dioxo-5-beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 3,12-dioxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum D1144/3 | 3beta,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+ | - |
[Clostridium] innocuum | 3beta,7alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+ | - |
? | |
1.1.1.391 | additional information | the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value | [Clostridium] innocuum | ? | - |
? | |
1.1.1.391 | additional information | the enzyme reduces a 3-oxo group in unconjugated and conjugated bile acids. Lower Km values are demonstrated with disubstituted than with trisubstituted bile acids, oxo functions at C-7 and C-12 further reduce the Km value | [Clostridium] innocuum D1144/3 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.391 | monomer | 1 * 56000, SDS-PAGE | [Clostridium] innocuum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.391 | 3beta-HSD | - |
[Clostridium] innocuum |
1.1.1.391 | NAD-dependent 3beta-hydroxysteroid dehydrogenase | - |
[Clostridium] innocuum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.391 | 37 | - |
assay at | [Clostridium] innocuum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.391 | 50 | - |
crude enzyme extract, pH 8.5, inactivation after 30 min | [Clostridium] innocuum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.391 | 10 | 10.02 | - |
[Clostridium] innocuum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.391 | NAD+ | dependent on | [Clostridium] innocuum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.1.1.391 | [Clostridium] innocuum | effect of bile acids on the production of 3beta-HSDH activity in Clostridium innocuum strain D1144/3 | down |