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Literature summary extracted from

  • Parascandolo, J.S.; Havemann, J.; Potter, H.K.; Huang, F.; Riva, E.; Connolly, J.; Wilkening, I.; Song, L.; Leadlay, P.F.; Tosin, M.
    Insights into 6-methylsalicylic acid bio-assembly by using chemical probes (2016), Angew. Chem. Int. Ed. Engl., 55, 3463-3467.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.165 expression in Escherichia coli Penicillium griseofulvum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.165 H958A mutation in the THID active site Penicillium griseofulvum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.165 Penicillium griseofulvum P22367
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.165 acetoacetyl-CoA + 3 malonyl-CoA + NADPH + H+
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 Penicillium griseofulvum 6-methyl triacetic acid lactone + CoA + NADP+
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2.3.1.165 acetyl-CoA + 3 malonyl-CoA + NADPH + H+
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 Penicillium griseofulvum 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O
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2.3.1.165 additional information no enzymatic dehydration takes place at a triketide stage of 6-MSA assembly, so loss of water must occur at the tetraketide stage. The enzyme is able to accept malonate surrogates of different chain lengths and bearing various functionalities (including alkyne and fluorine moieties) at different positions and for every round of chain extension, and also to generate novel pentaketide products Penicillium griseofulvum ?
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