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Literature summary extracted from

  • Abdoul Zabar, J.; Lorilliere, M.; Yi, D.; Thangavelu, S.; Devamani, T.; Nauton, L.; Charmantray, F.; Helaine, V.; Fessner, W.; Hecquet, L.
    Engineering a thermostable transketolase for unnatural conversion of (2S)-hydroxyaldehydes (2015), Adv. Synth. Catal., 357, 1715-1720.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 D477A the mutant shows a kcat/KM value decrease for (2R)-hydroxylated aldehydes decrease by three orders of magnitude compared to the wild type enzyme Geobacillus stearothermophilus
2.2.1.1 L382D/D470S the mutant leads up to 4 and 5fold higher activities towards L-lactaldehyde and L-glyceraldehyde, respectively, compared to the wild type enzyme Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 6
-
 D-glyceraldehyde at pH 7.2 and 50°C Geobacillus stearothermophilus
2.2.1.1 8.4
-
 D-Lactaldehyde at pH 7.2 and 50°C Geobacillus stearothermophilus
2.2.1.1 300
-
 L-glyceraldehyde Km above 300 mM, at pH 7.2 and 50°C Geobacillus stearothermophilus
2.2.1.1 300
-
 L-lactaldehyde Km above 300 mM, at pH 7.2 and 50°C Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Geobacillus stearothermophilus A0A0I9QGZ2
-
-

Storage Stability

EC Number Storage Stability Organism
2.2.1.1 50°C, 7 days, retains 100% activity Geobacillus stearothermophilus
2.2.1.1 65°C, 3 days, retains 100% activity Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-glyceraldehyde + hydroxypyruvate
-
 Geobacillus stearothermophilus D-ribulose + ?
-
 ?
2.2.1.1 D-lactaldehyde + hydroxypyruvate
-
 Geobacillus stearothermophilus 5-deoxy-D-ribulose + ?
-
 ?
2.2.1.1 L-glyceraldehyde + hydroxypyruvate minor reactivity Geobacillus stearothermophilus L-ribulose + ?
-
 ?
2.2.1.1 L-lactaldehyde + hydroxypyruvate minor reactivity Geobacillus stearothermophilus 5-deoxy-L-ribulose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.1 65
-
-
 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Geobacillus stearothermophilus