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Literature summary extracted from

  • Kim, J.; Kim, K.
    Purification, crystallization and preliminary X-ray diffraction analysis of 3-ketoacyl-CoA thiolase A1887 from Ralstonia eutropha H16 (2015), Acta Crystallogr. Sect. F, 71, 758-762.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9 recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Cupriavidus necator

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.9 purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 140 mg/ml protein in 40 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 17% PEG 8000, 0.1 M HEPES pH 7.0, and equilibration against 0.5 ml of reservoir solution, 20°C, 7 days, X-ray diffraction structure determination and analysis at 1.4 A resolution Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.158
-
 acetoacetyl-CoA pH 8.3, 30°C, recombinant enzyme Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.9 Mg2+ required Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 40000
-
 2 * 40000, recombinant His-tagged enzyme, SDS-PAGE Cupriavidus necator
2.3.1.9 80000
-
 recombinant His-tagged enzyme, gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 2 acetyl-CoA Cupriavidus necator
-
 CoA + acetoacetyl-CoA
-
 r
2.3.1.9 2 acetyl-CoA Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 CoA + acetoacetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA Cupriavidus necator
-
 2 acetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 2 acetyl-CoA
-
 r
2.3.1.9 additional information Cupriavidus necator the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA ?
-
 ?
2.3.1.9 additional information Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Cupriavidus necator Q0KAI3 gene H16_A1887; gene H16_A1887
-
2.3.1.9 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0KAI3 gene H16_A1887; gene H16_A1887
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography and gel filtration Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 2 acetyl-CoA
-
 Cupriavidus necator CoA + acetoacetyl-CoA
-
 r
2.3.1.9 2 acetyl-CoA
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 CoA + acetoacetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA
-
 Cupriavidus necator 2 acetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 2 acetyl-CoA
-
 r
2.3.1.9 additional information the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA Cupriavidus necator ?
-
 ?
2.3.1.9 additional information the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.9 homodimer 2 * 40000, recombinant His-tagged enzyme, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
2.3.1.9 3-ketoacyl-CoA thiolase
-
 Cupriavidus necator
2.3.1.9 A1887
-
 Cupriavidus necator
2.3.1.9 ACAT
-
 Cupriavidus necator
2.3.1.9 acetoacetyl-CoA thiolase
-
 Cupriavidus necator
2.3.1.9 KACT
-
 Cupriavidus necator
2.3.1.9 type II thiolase
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.9 30
-
 assay at Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.9 5000000
-
 acetoacetyl-CoA pH 8.3, 30°C, recombinant enzyme Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 8.3
-
 assay at Cupriavidus necator

General Information

EC Number General Information Comment Organism
2.3.1.9 metabolism the enzyme shows degradative thiolase activity catalyzing the fourth step of beta-oxidation degradative pathways by converting 3-ketoacyl-CoA to acyl-CoA Cupriavidus necator

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.9 31650
-
 acetoacetyl-CoA pH 8.3, 30°C, recombinant enzyme Cupriavidus necator