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Literature summary extracted from

  • Prokofev, I.I.; Lashkov, A.A.; Gabdulkhakov, A.G.; Dontsova, M.V.; Seregina, T.A.; Mironov, A.S.; Betzel, C.; Mikhailov, A.M.
    Crystallization and preliminary X-ray study of Vibrio cholerae uridine phosphorylase in complex with 6-methyluracil (2014), Acta Crystallogr. Sect. F, 70, 60-63.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3 expressed in Escherichia coli strain AM201 Vibrio cholerae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 in complex with 6-methyluracil, hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.1 M MgCl2, 0.1 M Tris-HCl pH 8.5 Vibrio cholerae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.3 27500
-
 6 * 27500, calculated from amino acid sequence Vibrio cholerae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Vibrio cholerae
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Vibrio cholerae Q9K4U1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3 ammonium sulfate precipitation, butyl Sepharose column chromatography, and Q Sepharose column chromatography Vibrio cholerae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 6-methyluridine + phosphate
-
 Vibrio cholerae 6-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Vibrio cholerae uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.3 homohexamer 6 * 27500, calculated from amino acid sequence Vibrio cholerae

Synonyms

EC Number Synonyms Comment Organism
2.4.2.3 UPH
-
 Vibrio cholerae