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Literature summary extracted from

  • Skov, L.K.; Pizzut-Serin, S.; Remaud-Simeon, M.; Ernst, H.A.; Gajhede, M.; Mirza, O.
    The structure of amylosucrase from Deinococcus radiodurans has an unusual open active-site topology (2013), Acta Crystallogr. Sect. F, 69, 973-978.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.4 recombinant expression in Escherichia coli strain BL21 Deinococcus radiodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.4 purified wild-type and mutant ligand-free enzyme, hanging drop vapour diffusion method, mixing of 0.0025 ml of 3.3 mg/ml protein in 50 mM HEPES, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT, with 0.0025 ml of reservoir solution containing 1 M sodium acetate, 0.1 M imidazole, 0.1 M LiCl, pH 6.5, 4°C, X-ray diffraction structure determination and analysis at 3.15 A resolution, modeling Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n Deinococcus radiodurans amylosucrases catalyze the formation of an alpha-1,4-glucosidic linkage by transferring a glucosyl unit from sucrose onto an acceptor alpha-1,4-glucan D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Deinococcus radiodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.4 recombinant enzyme from Escherichia coli strain BL21 to homogeneity using GST-affinity chromatography followed by dialysis and subsequent tag removal via PreScission protease and reverse affinity chromatography Deinococcus radiodurans

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n = D-fructose + [(1->4)-alpha-D-glucosyl]n+1 the elongation mechanism involves enzyme conformational changes to allow the entry of sucrose to the active site Deinococcus radiodurans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n amylosucrases catalyze the formation of an alpha-1,4-glucosidic linkage by transferring a glucosyl unit from sucrose onto an acceptor alpha-1,4-glucan Deinococcus radiodurans D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.4 More the putative enzyme interface is primarily mediated by two regions from domain N, residues Thr22, Leu25, Arg26, Arg29 and Tyr30 and residues Glu73, Leu76 and Leu77, which are involved in hydrophobic interactions with the corresponding residues from the second protomer Deinococcus radiodurans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.4 DRAS
-
 Deinococcus radiodurans

General Information

EC Number General Information Comment Organism
2.4.1.4 additional information ligand binding causes extensive changes in active-site topology Deinococcus radiodurans